Life Technologies 大家庭中的细胞培养产品。 Life Technologies公司已准备就绪，以满足您的细胞培养需要一个全面的选择，包括培养基，营养添加剂，盐溶液，缓冲液，化学物质，解离试剂和转染的试剂。您可以依靠严格的质量控制和，以保证您的满意。仅供研究使用。 不用于任何动物或人类治疗或诊断使用。
THERMO/LIFE Avidin/Biotin Blocking Kit货号004303 也被称为 00-4303描述This kit is used to block endogenous biotin or biotin-binding activity in tissue sections. The kit contains 18 mL each of Avid
详细介绍
THERMO/LIFE Avidin/Biotin Blocking Kit货号004303
也被称为 00-4303 描述 This kit is used to block endogenous biotin or biotin-binding activity in tissue sections. The kit contains 18 mL each of Avid
The MagMAX™ Cell-Free DNA Isolation Kit is designed for enrichment of circulating cell-free DNA (cfDNA) and optimized for use with biological samples such as serum and plasma. The kit is based on MagMAX™ magnetic-bead technology, enabling reproducible recovery of high-quality DNA that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing.
Scalable isolation of circulating nucleic acid from plasma, serum, and urine cfDNA recovery is challenging due to the limited amount present in cell-free samples. The MagMAX Cell-Free DNA Isolation Kit addresses this with its magnetic bead-based purification format which allows you to easily process a wide range of sample input volumes, from 500 µL to 10 mL. When used with either a KingFisher™ Duo Prime or KingFisher™ Flex magnetic particle processor, 6 to 24 plasma/serum samples of 2 mL input volume can be isolated at once. Alternatively, samples can be processed manually with the use of a magnetic stand.
Features of the MagMAX Cell-Free DNA Isolation Kit include:
• Flexible design that permits both manual and automated isolation of cfDNA • Scalable format that allows for higher and lower input volumes • Automation-ready, phenol-free extraction • Fast procedure, which allows 6 to 24 samples to be processed typically in 40 minutes or less when used with the KingFisher Duo Prime Magnetic Particle Processor or the KingFisher Flex Magnetic Particle Processor with a 24 Deep-Well Head • Wide sample volume inputs range from 500 µL to 10 mL of plasma/serum or urine • Contains Dynabeads™ MyOne™ Silane for consistent isolation of cfDNA • Elution volumes range from 15 µL to 50 µL
Life ivitrogen Gateway BP Clonase II 酶/BP酶Gateway® BP Clonase® II 酶混合物催化来自克隆的 PCR 产物或者亚克隆 DNA 片段（含有 attB 位点）与供体载体（含有 attP 位点）进行体外重组，产生入门克隆。 Gateway® BP Clonase® II 是包含酶和缓冲剂的单一型混合物，所需的移液步骤更少，因此可以快捷地设
详细介绍
Life ivitrogen Gateway BP Clonase II 酶/BP酶
描述
Gateway® BP Clonase® II 酶混合物催化来自克隆的 PCR 产物或者亚克隆 DNA 片段（含有attB 位点）与供体载体（含有 attP 位点）进行体外重组，产生入门克隆。 Gateway® BP Clonase® II 是包含酶和缓冲剂的单一型混合物，所需的移液步骤更少，因此可以快捷地设置十微升反应体系。
规格
Enzyme:
BP Clonase
Product Size:
20 reactions
Shipping Condition:
Dry Ice
内容及储存
Gateway® BP Clonase® II enzyme mix includes proteinase K solution (2 µg/µl) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored.
LIFE ambion trizol LS 200ml货号10296-028规格：200mlTRIzol® LS Reagent 是苯酚和异硫氰酸胍的即用型单相溶液，设计用于从人、动物、植物、酵母、细菌和病毒来源的液体样品中分离总 RNA。 在样品匀浆或裂解过程中，TRIzol® LS Reagent 一方面保持 RNA 的完整性，另一方面破坏细胞并裂解细胞组分。
详细介绍
LIFE ambion trizol LS 200ml货号10296-028
TRIzol® LS 试剂分离的总 RNA 不含蛋白质和 DNA 杂质。 可用于 Northern blot 分析，点印迹杂交，多聚 (A)+ 选择，体外翻译，RNA 酶保护分析，RT-PCR 和分子克隆。 对于 RT-PCR，当一个外显子中存在两个引物时， 建议用扩增级 DNase I（商品目录号 18068-015）处理分离的 RNA。
TRIzol® LS Reagent 的特点： • 设计用于液体样品，例如血清和病毒制品 • 使用 TRIzol® LS 试剂与液体样品的比值为 3:1 • 有助于多种大分子或小分子 RNA 的分离
LIFE ambion trizol LS 200ml货号10296-028
述
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour.
• Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein from a single liquid sample • Offers excellent lysis capability, even with difficult biological fluids
Reliably Purify RNA from Multiple Sample Sources TRIzol® LS Reagent is designed for processing a variety of liquid samples of up to 0.25 ml in volume. TRIzol® LS Reagent differs from the standard TRIzol® Reagent in concentration, which permits larger samples to be processed. Just as with the standard TRIzol® Reagent, the integrity of resulting RNA preparations is maintained by the highly effective inhibition of RNase activity during sample homogenization. The simplicity of the TRIzol® LS Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol® LS Reagent is free of protein and DNA contamination.
Formulated to Serve Multiple Isolations TRIzol® LS Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® LS Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interface, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the aqueous/organic interface with ethanol. Protein is precipitated from the phenol-ethanol layer by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.
Purified Products are Ideal for Use with a Variety of Applications Isolated RNA can be used in real-time quantitative PCR (qPCR), northern blot analysis, dot blot hybridization, poly-(A)+ selection, In vitro translation, RNase protection assays, and molecular cloning. Isolated DNA can be used in PCR, restriction enzyme digestion, and Southern blots. Isolated protein can be used for western blots, recovery of some enzymatic activity, and someimmunoprecipitation.
Gibco® Sera: Trusted Quality for Consistent Performance
Sera Category: Performance Plus • Use for a broad range of cell types, especially sensitive cell lines • Our most characterized sera with the lowest endotoxin and hemoglobin levels • Endotoxin level: ≤ 5 EU/ml • Hemoglobin level: ≤ 10 mg/dl • Origin: United States
Gibco® Serum Delivers • ISO 13485 certified, processed in FDA registered facilities • Labeled for in-vitro diagnostics (compliant with highest level of USP sterility testing) • Triple filtered at 0.1 µm • Gibco® bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides:
• Rapid, non-hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications
Rapid and convenient column purification The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 µg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Simplified, nontoxic RNA purification The PureLink® RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.
The PureLink RNA Mini Kit is recommended to be used together with the Homogenizer (Cat. No. 12183026) designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.
For plant tissues rich in polyphenolics or starch (e.g., pine needles, potato tubers), we recommend using PureLink™ Plant RNA Reagent (Cat. No. 12322012) together with the PureLink RNA Mini Kit.
LIFE公司（代理）磁珠Dynabeads® His-Tag Isolation and Pulldown货号： 10103D规格：2ml
详细介绍
LIFE公司（代理）磁珠
描述
This product is designed for his-tagged protein isolation. High selectivity for poly-histidine tags results in extremely low background levels.
The new bead surface chemistry is Cobalt-based and offers exceptionally fast binding kinetics and a high yield per microliter of beads. The entire protocol (from cleared lysate to purified protein) takes only 20 minutes! The protocol includes instructions for making the required buffers as well as methods for optimizing buffer composition.
Dynabeads® His-Tag Isolation & Pulldown replaces Dynabeads® TALON™. Dynabeads® His-Tag Isolation & Pulldown has a new optimized binding chemistry that offers significant advantages over Dynabeads® TALON™ which were coated in the TALON™ chemistry. This optimization results in a cleaner prep with higher yield of his-tag protein and extremely low background levels.
Dynabeads His-Tag Isolation & Pulldown is designed for isolating His-tagged proteins from cleared lysates. Isolated his-tagged proteins can either be eluted from the beads or kept on the beads for use in bead-based downstream assays. Bead bound his-tagged proteins can be used in various downstream assays such as bait-prey pulldowns, etc.
Note: This product contains Dynabeads and a protocol only, and does not include buffers.
The technology used for the discontinued Dynabeads® TALON™ was licensed from Clontech. TALON™ is a registered trademark of Clontech Laboratories Inc., USA.
LIFE RNase ZAP AM9780Reagent Type:RNase Control ReagentForm:Liquid规格:250 mLShipping Condition:Room Temperature
详细介绍
LIFE RNase ZAP AM9780
RNaseZap? RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap? Solution onto the surface to be decontaminated and rinse it off with RNase-free water.
LIFE RNase ZAP AM9780
eatures of RNaseZap? RNase Decontamination Solution:
? Compley removes RNase contamination from glass and plastic surfaces ? Excels at removing high levels of RNase contamination whereas similar products fail ? Proven effective at removing high concentrations of dried-on RNase A ? Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free
Using RNaseZap? Solution Working with RNA requires that special measures be taken to ensure an RNase-free environment. Even trace quantities of RNase can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results in RPAs and Northerns. RNaseZap? contains three ingredients active against RNase and has proven to be extremely effective at removing RNase contamination from glassware, plastic surfaces, countertops, and pipettors. It has also been shown to be effective at eliminating RNase contamination from microfuge tubes without inhibiting subsequent enzymatic reactions. Supplied in one 250 mL bottle.
