This is a 47 mm hydrophilic Glass Fiber with Binder Resin filter with a 2.0 um ( 2 micron ) filter pore size. The 2 micron hydrophilic glass fiber filter is a recommended prefilter for 0.9 to 8um filters, especially for proteinaceious & heavily contaminated liquids.
GE PD Spintrap G-25微量样品脱盐层析柱/28-9180-04Z大特点:特别适合样本量体积较小的样本,避免了样本的浪费规格:50支/盒
详细介绍
GE PD Spintrap G-25微量样品脱盐层析柱/28-9180-04
zui大特点:所需样本体积很小
Sample Volume
100-180 μl
GE PD Spintrap G-25微量样品脱盐层析柱/28-9180-04
PD SpinTrap G-25
PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules
Convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr) with the possibility to run multiple samples in parallel.
Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
High desalting capacity.
Reproducible, high throughput, parallel cleanup of protein samples.
Sample preparation in the range of 100 to 180 μl without sample dilution.
PD SpinTrap G-25 is a single-use microspin column that is designed for desalting and buffer exchange of biological sample using a standard microcentrifuge. The column is prepacked with ~600 μl Sephadex G-25 Medium, and the column material is biocompatible polypropylene.
Instructions supplied with the columns
详细参数:
PD SpinTrap G-25
Certificate of Analysis
Yes
Sample Volume
100-180 μl
Complete Packsize
50 Columns
Storage Conditions
4 to 30°C, 0,05% Kathon CG/ICP Biocide
Pressure Max. [Over the Packed Bed During Operation]
N/A (N/A)
Wetted Part
No
Media
BioProcess resin
Yes
Matrix
Cross-linked dextran
pH Stability Working Range
2–13
pH Stability Cleaning
2–13
pH stability, CIP
2–13
Pressure/Flow Specification
>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.
Exclusion Limit [Mr] [Globular Proteins]
> 5 x 103
Exclusion Limit
Mr 5000
Chemical Stability
All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
dako IgM, Specific for Mu-Chains (Multipurpose)现货主要功能Excellent specificity and precipitation abilityHigh lot to lot consistencyOptimized for a variety of applications with validated protocolsCer
详细介绍
dako IgM, Specific for Mu-Chains (Multipurpose)现货
免疫原
IgM isolated from human serum of a patient with Waldenström's macroglobulinaemia.
dako IgM, Specific for Mu-Chains (Multipurpose)现货
特异性
The antibody reacts with the mu-chains of human IgM. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
In crossed immunoelectrophoresis, using 12.5 µL antibody per cm2 gel area against 2 µL human plasma, only one precipitate corresponding to IgM appears. Staining: Coomassie Brilliant Blue.
In indirect ELISA no significant reaction is observed when using human IgG and IgA as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgM.
Cross-reaction with IgM from other species may occur. As demonstrated by rocket immunoelectrophoresis, the antibody cross-reacts with the IgM-equivalent protein in cat, cow, deer, dog, elephant, hamster, horse, mink, mouse, polecat, raccoon dog, rat, seal, sheep and swine (9).
dako IgM, Specific for Mu-Chains (Multipurpose)现货
试剂提供
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
Protein concentration g/L: See label on vial. Antibody titre (SRI): 5300 mg/L (8).
Dako Human Serum Protein Calibrator, Code X0908, was used as reference for the titre determination. The titre variation between different lots of A 0425 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
Nitrocellulose blotting membrane, with 0.45 µm pore size, provides excellent sensitivity, resolution, and low background in fluorescence Western blotting detection applications.
High binding capacity (162 – 180 µg IgG/cm2).
Suitable for proteins of a wide range of molecular weights.
Unsupported, 100% pure nitrocellulose membrane for use with all ECL Western blotting systems and protein blotting applications.
Available in popular precut sizes for your application.
Amersham Protran Premium offers excellent sensitivity, resolution, and low background, especially when used with the fluorescence Amersham ECL Plex Western Blotting Detection System.
For convenience, Western blotting sandwiches (10 sandwiches/package) are available, consisting of precut Amersham Protran Premium 0.45 NC membranes preassembled with 2 x 3MM Chr Blotting papers.
GE Amersham蛋白转印迹膜(NC膜)0.45um型号10600002孔径:0.45um现货供应:替代10401196
详细介绍
GE Amersham蛋白转印迹膜(NC膜)0.45um型号10600002
Amersham Protran 0.45 NC 300mm×4m 1 roll/PK
Nitrocellulose membrane, with a 0.45 µm pore size, is suitable for proteins of a wide range of molecular weights.
Low background and excellent signal-to-noise ratios.
Compatible with a variety of labeling and detection systems.
High binding capacity.
Suitable for proteins of a wide range of molecular weights.
Proprietary nitrocellulose formula gives a long shelf life of bound proteins.
Available in popular precut sizes for your application.
Amersham Protran offers excellent sensitivity, resolution, and low background, especially when used with Amersham ECL, Amersham ECL Prime, or Amersham ECL Select Western Blotting Systems.
For convenience, Western blotting sandwiches (10 sandwiches/package) are available, consisting of precut Amersham Protran 0.45 NC membranes preassembled with 2 x 3MM Chr Blotting papers.
参考文献 1. Brown JA, Dorfman DM, Ma F-R, Sullivan EL, Munoz O, Wood CR, et al. Blockade of Programmed Death-1 Ligands on Dendritic Cells Enhances T Cell Activation and Cytokine Production. J Immunol 2003;170:1257. 2. Ellison AR. Technical Report: Characterization of monoclonal mouse anti-PD-L1 antibody clone 22C3. D29539/rev01/C22282, 2015
Nitrocellulose membrane, with a 0.2 µm pore size and high surface area, for binding of small proteins (< Mr 20 000).
Low background and excellent signal-to-noise ratios, without the need for stringent washing conditions. Compatible with a variety of labeling and detection systems.
High binding capacity of small proteins (< Mr 20 000) reduces risk of “blow-through”.
Proprietary nitrocellulose formula gives a long shelf life of bound proteins.
Available in popular precut sizes for your application.
Amersham Protran offers excellent sensitivity, resolution, and low background, especially when used with Amersham ECL, Amersham ECL Prime, or Amersham ECL Select Western Blotting Systems.
For convenience, Western blotting sandwiches (10 sandwiches/package) are available, consisting of precut Amersham Protran 0.20 NC membranes preassembled with 2 x 3MM Chr Blotting papers.