LIFE公司（代理）磁珠Dynabeads® His-Tag Isolation and Pulldown货号： 10103D规格：2ml
详细介绍
LIFE公司（代理）磁珠
描述
This product is designed for his-tagged protein isolation. High selectivity for poly-histidine tags results in extremely low background levels.
The new bead surface chemistry is Cobalt-based and offers exceptionally fast binding kinetics and a high yield per microliter of beads. The entire protocol (from cleared lysate to purified protein) takes only 20 minutes! The protocol includes instructions for making the required buffers as well as methods for optimizing buffer composition.
Dynabeads® His-Tag Isolation & Pulldown replaces Dynabeads® TALON™. Dynabeads® His-Tag Isolation & Pulldown has a new optimized binding chemistry that offers significant advantages over Dynabeads® TALON™ which were coated in the TALON™ chemistry. This optimization results in a cleaner prep with higher yield of his-tag protein and extremely low background levels.
Dynabeads His-Tag Isolation & Pulldown is designed for isolating His-tagged proteins from cleared lysates. Isolated his-tagged proteins can either be eluted from the beads or kept on the beads for use in bead-based downstream assays. Bead bound his-tagged proteins can be used in various downstream assays such as bait-prey pulldowns, etc.
Note: This product contains Dynabeads and a protocol only, and does not include buffers.
The technology used for the discontinued Dynabeads® TALON™ was licensed from Clontech. TALON™ is a registered trademark of Clontech Laboratories Inc., USA.
CST Anti-GFP （D5.1） XP抗体Isotype：Rabbit IgGApplications：W IHC-P IF-IC FReactivity：AllSensitivity：Transfected Only
详细介绍
CST Anti-GFP （D5.1） XP抗体
Specificity / Sensitivity
GFP （D5.1） XP® Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.
Western Blotting
Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP （D5.1） XP® Rabbit mAb.
IHC-P （paraffin）
Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected （left） or GFP-transfected （right）, using GFP （D5.1） XP® Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HCC827 cells, untransfected （blue） or GFP-transfected （green）, using GFP （D5.1） XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of COS-7 cells expressing a GFP-tagged fusion protein （green） using GFP （D5.1） XP® Rabbit mAb （red）. Blue pseudocolor = DRAQ5® #4084 （fluorescent DNA dye）.
CST Anti-GFP （D5.1） XP抗体
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The green-fluorescent protein （GFP） functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization （1）.
Miyawaki, A. et al. （2003） Curr Opin Chem Biol 7, 557-62.
Application References
Matheny, R.W. and Nindl, B.C. （2011） Endocrinology 152, 1923-34. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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2372 β-Gal （14B7） Mouse mAb
2367 HA-Tag （6E2） Mouse mAb
2365 His-Tag Antibody
2276 Myc-Tag （9B11） Mouse mAb
7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074 Anti-rabbit IgG, HRP-linked Antibody
7720 Prestained Protein Marker, Broad Range （Premixed Format）
7727 Biotinylated Protein Ladder Detection Pack
7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
