This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining.
RESEARCH DIETS代理MCD饲料Methionine Choline Deficient DietMCD造模饲料是蛋氨酸胆碱缺乏的造模饲料（Methionine- and Choline-Deficent Diet），其对照饲料是MCS饲料（即蛋氨酸胆碱正常含量的饲料, Methionine- and Choline-Supplement Diet）。
详细介绍
RESEARCH DIETS代理MCD饲料Methionine Choline Deficient Diet
规格：12.5kg/箱
RESEARCH DIETS代理MCD饲料Methionine Choline Deficient Diet
Nagasawa T, Inada Y, Nakano S, Tamura T, Takahashi T, Maruyama K, Yamazaki Y, Kuroda J, Shibata N. Effects of bezafibrate, PPAR pan-agonist, and GW501516, PPARdelta agonist, on development of steatohepatitis in mice fed a methionine- and choline-deficient diet. Eur J Pharmacol 2006;536:182-91
描述 产品目录编号 NN15H04700 描述 尼龙网膜，亲水，150 µm，47 mm 产品信息 过滤器代码 NY6H 过滤器颜色 白色 zui高工作温度 100 °C 过滤器类型 网格膜 应用 应用 This is a 47 mm Hydrophilic Nylon Net Filter with a 160.0 µm pore size. For use in Collection of algae & cells, particle analysis, large particulate filtration, prefiltration of solvents, paint monitoring.
Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) HRP substrate for low-picogram-level detection by Western blot analysis.
SuperSignal West Pico Substrate provides intense signal and picogram sensitivity for Western blotting with horseradish peroxidase (HRP) enzyme conjugates. The ECL substrate is compatible with different membranes, blocking reagents and a wide range of antibody dilutions. SuperSignal West Pico Substrate provides excellent performance, versatility and economy for routine Western blotting needs.
Features of SuperSignal West Pico Chemiluminescent Substrate:
• ECL—enhanced chemiluminescent substrate for horseradish peroxidase (HRP) • Picogram sensitivity—detect low-picogram amounts of protein in bands on nitrocellulose or PVDF membrane • Good signal duration—incubated blots provide 6 to 8 hours of usable light output when conditions are optimized • Stable reagent—24-hour working solution stability; 1-year kit stability at room temperature • Economical—optimized for dilute antibody concentrations: • 0.2 to 1.0 µg/mL primary antibody (1:1000 to 1:5000 dilution from 1 mg/mL stock) • 10 to 50 ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/mL stock)
THERMO/pierce化学发光底物（皮克级）34077 34080
内容及储存
Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane • Luminol/Enhancer, 50 mL • Stable Peroxide Buffer, 50 mL
Gibco™ KnockOut™ Serum Replacement (KnockOut™ SR) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this serum replacement has been trusted by many for almost 20 years. Providing the documents required for regulatory review, KnockOut SR reduces your burden in qualifying reagents when transitioning from research applications to clinical applications. If you are interested in only basic research applications, see the Research Use Only version of this product to ensure that you receive the benefits and pricing that fit your needs.
With KnockOut Serum Replacement, you can: • Culture feeder-dependent PSCs with confidence • Rely on the improved performance of a qualified, FBS-free formulation • Enjoy the peace of mind of having a more stable price and supply compared to FBS
Culture PSCs with confidence KnockOut SR has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.
