QIAGEN RNase-Free DNase Set保证无RNA酶、经严格质控和优化，配合RNeasy操作流程和QIAamp RNA Blood Mini操作流程使用。一般来说，使用RNeasy Kits纯化RNA时，不需要DNA酶消化步骤，因为硅胶膜离心柱技术可有效地去除大部分DNA，而无需DNA酶处理。然而，某些RNA应用对痕量DNA非常敏感，因此需要*去除DNA。QIAGEN RNase-Free DNase Set中提供RDD缓冲液，经优化可用于柱上DNA酶消化。该缓冲液也非常适合于溶液中高效DNA酶消化。在使用RNeasy试剂盒和QIAamp RNA Blood Mini Kit从细胞和组织中纯化RNA的过程中，QIAGEN RNase-Free DNase Set提供高效的柱上DNA消化。后续的洗涤步骤可将DNA酶高效去除。当使用RNeasy 96 试剂盒过程中需要DNA酶处理时，请QIAGEN Technical Services或当地经销商获取专门优化的实验方案。QIAGEN RNase-Free DNase Set是以稳定的、冻干粉酶形式提供。QIAGEN RNase-Free DNase Set提供1500 Kunitz units。1 Kunitz units是指在25°C、pH 5.0条件下，以高度聚合的DNA为底物，使每毫升底物的A260值每分钟升高0.001时所需要的DNase I的量（Kunitz, M. [1950] Crystalline desoxyribonuclease. I. Isolation and general properties, spectrophotometric method for the measurement of desoxyribonuclease activity. II. Digestion of thymus nucleic acid (desoxyribonucleic acid): the kinetics of the reaction. J. Gen. Physiol. 33, 349 and 363）。
订购信息：
Cat No./ID: 79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps
The MagMAX™ Cell-Free DNA Isolation Kit is designed for enrichment of circulating cell-free DNA (cfDNA) and optimized for use with biological samples such as serum and plasma. The kit is based on MagMAX™ magnetic-bead technology, enabling reproducible recovery of high-quality DNA that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing.
Scalable isolation of circulating nucleic acid from plasma, serum, and urine cfDNA recovery is challenging due to the limited amount present in cell-free samples. The MagMAX Cell-Free DNA Isolation Kit addresses this with its magnetic bead-based purification format which allows you to easily process a wide range of sample input volumes, from 500 µL to 10 mL. When used with either a KingFisher™ Duo Prime or KingFisher™ Flex magnetic particle processor, 6 to 24 plasma/serum samples of 2 mL input volume can be isolated at once. Alternatively, samples can be processed manually with the use of a magnetic stand.
Features of the MagMAX Cell-Free DNA Isolation Kit include:
• Flexible design that permits both manual and automated isolation of cfDNA • Scalable format that allows for higher and lower input volumes • Automation-ready, phenol-free extraction • Fast procedure, which allows 6 to 24 samples to be processed typically in 40 minutes or less when used with the KingFisher Duo Prime Magnetic Particle Processor or the KingFisher Flex Magnetic Particle Processor with a 24 Deep-Well Head • Wide sample volume inputs range from 500 µL to 10 mL of plasma/serum or urine • Contains Dynabeads™ MyOne™ Silane for consistent isolation of cfDNA • Elution volumes range from 15 µL to 50 µL
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