Shibayagi 小鼠/大鼠 高分子量脂联素 ELISA试剂盒

Shibayagi 小鼠/大鼠 高分子量脂联素 ELISA试剂盒
Lbis® High Molecular Adiponectin-Mouse/Rat

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Lbis® High Molecular Adiponectin-Mouse/RatShibayagi 小鼠/大鼠 高分子量脂联素 ELISA试剂盒

Shibayagi 小鼠/大鼠 人高分子量脂联素 ELISA试剂盒

Shibayagi 小鼠/大鼠 高分子量脂联素 ELISA试剂盒

Adiponectin(脂联素)是脂肪细胞分泌的一种细胞因子。作为脂肪细胞因子,控制脂肪代谢和胰岛素感受性、是抗糖尿病、抗动脉粥样硬化、抗炎症的重要物质。血液中的脂联素通过聚集单聚体形成3聚体、6聚体或者是12-18聚体。三聚体(LMW)通过胶原三螺旋链的非共价相互作用以及球状体C1q域的疏水相互作用形成。三聚体聚集形成六聚体(MMW)或者更大的多聚体(HMW)。

Adiponectin与各种各样的生长因子相结合有明显的亲和性,将其隔离能影响细胞的生长、血管新生和细胞组织的重建。血液中HMW的测定值除了表示总脂联素以外,同时明确地反映出BMI和性别、体重减轻的影响、糖耐量、肝脏的胰岛素感受性、代谢综合征和2型糖尿病。预计HMW的测定比起总脂联素的测定,对于代谢综合征和DM2的分析更有帮助。

Shibayagi的此款试剂盒只用于测定高分子Adiponectin。

◆特点

 

● 短时间测定(总的反应时间:4小时)

● 微量样品可测

● 使用对环境无害的防腐剂

● 全部试剂均为液体,可直接使用

● 精密的测定精度和高再现性

 

 

◆构成

 

组成部分

状态

容量

(A)   抗体固相化 96 孔板

洗净后使用

96 wells(8×12)/1块

(B)   标准溶液(2,000 ng/mL)

稀释后使用

200 μL/1 瓶

(C)   缓冲液

即用

60 mL/1 瓶

(D)   HRP标识抗脂联素抗体

稀释后使用

100 μL/1 瓶

(F)   显色液(TMB)

即用

12 mL/1 瓶

(H)   反应终止液(1M H2SO4)※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液(10×)

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

◆交叉反应

动物种类

对象物质

反应性和交叉率(%)

Mouse

Adiponectin(HMW)

100

Adiponectin(Hexamer)

<5

Adiponectin(Trimer)

不存在交叉反应

Adiponectin(Monomer)

不存在交叉反应

MCH

不存在交叉反应

TNF-α

不存在交叉反应

IFN-γ

不存在交叉反应

Insulin

不存在交叉反应

Leptin

不存在交叉反应

Rat

Adiponectin(HMW)

100

Adiponectin(Monomer)

不存在交叉反应

TNF-α

不存在交叉反应

IFN-γ

不存在交叉反应

Insulin

不存在交叉反应

Leptin

不存在交叉反应

※交叉率浓度为1,000 ng/mL

◆样品信息

小鼠/大鼠的血清•血浆•培养液

50 μL/well(稀释样品)

※血浆采血建议使用肝素处理

※正常样品的稀释倍数为50倍(~25倍)

 

◆测量范围

3.13~200 ng/mL(标准曲线范围)

78.25~5,000 ng/mL(25倍稀释样品)

0.1565~10 μg/mL(50倍稀释样品)

 

◆Validation data

精度测试(检测内变异系数)

样品

A

B

1

29.5

129

2

30.7

125

3

29.8

128

4

29.0

126

5

29.6

126

mean

29.7

127

SD

0.631

1.89

CV(%)

2.12

1.49

单位:ng/mL

再现性测试(检测内变异系数)

测量日/样品

C

D

E

第0天

196

126

62.5

第1天

192

130

59.1

第2天

196

125

60.7

第3天

190

125

60.3

mean

193

127

60.7

SD

2.63

2.27

1.41

CV(%)

1.36

1.79

2.33

单位:ng/mL n=2

添加回收测试

样品H

添加量

实测值

回收量

回收率(%)

0

68.5

35.0

103

34.5

98.6

65.0

132

63.5

97.7

95.0

165

96.9

102

单位:ng/mL n=2



样品I

添加量

实测值

回收量

回收率(%)

0

23.3

18.0

40.3

17.0

94.4

26.0

50.6

27.3

105

32.0

55.5

32.2

101

单位:ng/mL n=2

 

稀释直线性测试

用稀释缓冲液分3次连续稀释2个血清样品的测量结果,直线回归方程的R2在0.9987~0.9993之间。

相关资料


Shibayagi 小鼠/大鼠 高分子量脂联素 ELISA试剂盒 Shibayagi 小鼠/大鼠 高分子量脂联素 ELISA试剂盒 Shibayagi 小鼠/大鼠 高分子量脂联素 ELISA试剂盒
说明书

ELISA试剂盒选择指南①②

ELISA试剂盒选择指③④

参考文献



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产品编号 产品名称 产品规格 产品等级 产品价格
638-13079 (AKMAN-011)Lbis® Mouse/Rat HMW Adiponectin ELISA Kit
Lbis® 小鼠/大鼠 人高分子量脂联素 ELISA试剂盒
96 tests