QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒12381

QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒

  • 产品型号:  12381
  • 简单描述
  • QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒EndoFree Plasmid Kits基于阴离子交换技术,可快速制备不含内毒素的质粒DNA。QIAfilter Cartridges过滤快速澄清裂解液。制备的DNA纯度超过两次CsCl梯度离心法所得DNA的纯度,适合超转染级纯的应用。EndoFree Plasmid Buffer Set可用于制备10次m
详细介绍

QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒

纯化得到至多10 mg的无内毒素高转染级纯质粒或柯斯质粒DNA

  • 纯化得到的DNA内毒素水平低于0.1 EU/µg
  • 纯化流程快,同时去除内毒素
  • 获得高产量、高拷贝质粒DNA
  • 使用LyseBlue,获得的裂解和高产量的DNA

QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒

质粒纯化方法与转染效率。

使用两种方法独立制备pRSVcat,分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中,及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit(100%)制备的DNA的转染效率表示。

质粒纯化方法与转染效率。

使用两种方法独立制备pRSVcat,分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中,及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit(100%)制备的DNA的转染效率表示。

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