| 1. AAV病毒提取试剂 |
|
| AAV Extraction Solution A |
1.5 ml×2 |
| AAV Extraction Solution B |
300 μl |
| 2. AAV基因组简易提取 (100 samples用) |
|
| DNase I |
100 μl |
| 10X DNase I Buffer |
200 μl |
| Lysis Buffer |
1 ml×2 |
| 3. Real Time PCR (25 μl体系×100次量) |
|
| TB Green Premix Ex Taq II (Tli RNaseH Plus)*1 |
625 μl×2 |
| AAV Forward Titer Primer |
20 μl |
| AAV Reverse Titer Primer |
20 μl |
| dH2O*2 |
1 ml×3 |
| ROX Reference Dye*3 |
50 μl |
| ROX Reference Dye II*3 |
50 μl |
| Positive Control |
50 μl |
| EASY Dilution (for Real Time PCR) |
1 ml×5 |
*1 试剂名称已更改。
*2 dH2O也用于DNase I反应中。
*3 使用在Applied Biosystems的Real Time PCR扩增仪上，用以校正孔与孔之间产生的荧光信号误差。StepOnePlus Real-Time PCR System使用ROX Reference Dye；Applied Biosystems 7500 Fast Real-Time PCR System使用ROX Reference Dye II。Thermal Cycler Dice Real Time System系列(Code No.: TP900/TP960：终卖)等Real Time PCR扩增仪时不必使用。 |
| |
| Limited Use Label License：[M82], [M84], [M85] |
