qiagen 74136DNA微量提取试剂盒
- 产品型号：
- 简单描述
- qiagen 74136DNA微量提取试剂盒应用RNeasy Plus Kit纯化得到的RNA适合对少量DNA污染敏感的下游应用，如定量real-time RT-PCR和RNA-Seq。纯化得到的RNA还可用于其他应用。RNeasy Plus Micro Kit适用于小样本，包括：激光显微切割冰冻切片 细针穿刺
qiagen 74136DNA微量提取试剂盒
操作流程
RNeasy Plus Micro Kit能从多达5 x 105个细胞或5 mg组织中分离总RNA，RNeasy Plus Mini Kit能够从107个细胞或30 mg组织中分离总RNA。简洁的工作流程可在25分钟内纯化得到已去除基因组DNA的RNA。样本首先被裂解和匀质化。裂解液流过gDNA Eliminator柱，在流过gDNA Eliminator柱的溶液中加入乙醇，样本再上样到RNeasy离心柱。RNA结合到膜上，污染物被洗去。获得高品质RNA，RNeasy Plus Micro Kit的洗脱体积为14 µl，RNeasy Plus Mini Kit的洗脱体积为30 µl。
处理不同的起始样本可用不同的实验方案。实验方案的不同之处主要在于样本的裂解和匀质化。样本上样到gDNA Eliminator柱之后的步骤就类似。
使用Buffer RLT Plus（RNeasy Plus Micro Kit提供）进行组织破碎和匀质化时，可能会产生过多泡沫。在破碎和匀浆前向Buffer RLT Plus中加入Reagent DX（单独提供）可充分减少泡沫产生。Reagent DX经仔细检测，表明配合此试剂盒使用不会影响RNA的纯度或下游应用。
qiagen 74136DNA微量提取试剂盒
For purification of up to 100 µg total RNA from cells/tissues using gDNA Eliminator columns
- Unique gDNA Eliminator columns avoid the need for DNase
- Efficient removal of genomic DNA
- Highly reproducible yields of RNA in minutes
- High-performance RNA for sensitive applications
The RNeasy Plus Mini Kit integrates fast, convenient purification of up to 100 µg RNA with effective elimination of genomic DNA. Cell or tissue lysates are spun through gDNA Eliminator spin columns to remove genomic DNA. Total RNA is then purified using RNeasy Mini spin columns. The kit can be automated on theQIAcube. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For smaller samples, the RNeasy Plus Micro Kit (spin-column binding capacity of 45 µg RNA) is also available.
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