qiagen 12145质粒提取试剂盒Plasmid Midi Kit

qiagen 12145质粒提取试剂盒Plasmid Midi Kit

  • 产品型号:  
  • 简单描述
  • qiagen 12145质粒提取试剂盒Plasmid Midi KitQIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多10 mg的质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。
详细介绍

qiagen 12145质粒提取试剂盒Plasmid Midi Kit

转染效率与质粒纯化方法。

采用不同方法制备pRSVcat DNA,通过脂质体介导的转染将其导入的细胞系内,40小时后测定CAT表达水平,确定转染效率。每个柱形代表4个独立的转录的平均值(2个质粒制备物分别转染2次)。使用QIAGEN Plasmid Kit可获得zui高的转染效率。

qiagen 12145质粒提取试剂盒Plasmid Midi Kit

Performance

QIAGEN Plasmid Kit利用重力流QIAGEN阴离子交换柱技术高效纯化质粒DNA。产量可达10 mg(Giga)、2.5 mg(Mega)、500 µg(Maxi)、或100 µg(Midi)高拷贝质粒DNA(培养体积取决于质粒拷贝数、插入片段大小、宿主菌株和培养基。)QIAGEN Plasmid Kits纯化的质粒DNA适合多种应用,如:转染(参见”Transfection efficiency versus plasmid purification method”)、克隆和体外转录。

Principle

QIAGEN-tips内*的阴离子交换树脂专为纯化核酸设计。它*的分离特性,使纯化的DNA的纯度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套QIAGEN质粒纯化体系避免苯酚、lvfang、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

Procedure

使用QIAGEN Plasmid Kits,细菌裂解物通过离心澄清。澄清后的裂解物上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、代谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯质粒DNA(参见”QIAGEN Plasmid Kit procedures”)。得到的DNA用异丙醇沉淀浓缩和脱盐后,离心收集。

Applications

QIAGEN Plasmid Kits纯化质粒DNA适合多种应用,如:

转染

克隆

PCR

体外转录

应用 Transfection, cloning, sequencing, capillary sequencing etc.
起始样本体积 2.5–5 liters culture volume
质粒类型 High-copy, low-copy, cosmid DNA
处理 Manual (centrifugation)
每次处理样本量;通量 1 sample per run
技术 Anion-exchange technology
每次运行时间或处理样本数 320 min
产量 <10 mg

订购信息:

 

QIAGEN Plasmid Midi Kit (25) 3120 12143 25次
QIAGEN Plasmid Midi Kit (100) 11510 12145 100从

 

qiagen质粒中提试剂盒Plasmid Midi Kit型号12143

qiagen质粒中提试剂盒Plasmid Midi Kit型号12143

  • 产品型号:  
  • 简单描述
  • qiagen质粒中提试剂盒Plasmid Midi Kit型号12143纯化至多10 mg转染级质粒或柯斯质粒DNA纯化得到相当于连续2次CsCl梯度离心得到的DNA纯度高产量质粒DNA经济的制备方法使用LyseBlue,获得的裂解效果和Z大的DNA产量
详细介绍

qiagen质粒中提试剂盒Plasmid Midi Kit型号12143

QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多10 mg的质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。

qiagen质粒中提试剂盒Plasmid Midi Kit型号12143

转染效率与质粒纯化方法。

采用不同方法制备pRSVcat DNA,通过脂质体介导的转染将其导入的细胞系内,40小时后测定CAT表达水平,确定转染效率。每个柱形代表4个独立的转录的平均值(2个质粒制备物分别转染2次)。使用QIAGEN Plasmid Kit可获得zui高的转染效率。

Performance

QIAGEN Plasmid Kit利用重力流QIAGEN阴离子交换柱技术高效纯化质粒DNA。产量可达10 mg(Giga)、2.5 mg(Mega)、500 µg(Maxi)、或100 µg(Midi)高拷贝质粒DNA(培养体积取决于质粒拷贝数、插入片段大小、宿主菌株和培养基。)QIAGEN Plasmid Kits纯化的质粒DNA适合多种应用,如:转染(参见”Transfection efficiency versus plasmid purification method”)、克隆和体外转录。

Principle

QIAGEN-tips内*的阴离子交换树脂专为纯化核酸设计。它*的分离特性,使纯化的DNA的纯度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套QIAGEN质粒纯化体系避免苯酚、lvfang、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

Procedure

使用QIAGEN Plasmid Kits,细菌裂解物通过离心澄清。澄清后的裂解物上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、代谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯质粒DNA(参见”QIAGEN Plasmid Kit procedures”)。得到的DNA用异丙醇沉淀浓缩和脱盐后,离心收集。

Applications

QIAGEN Plasmid Kits纯化质粒DNA适合多种应用,如:

转染

克隆

PCR

体外转录

订购信息:

 

QIAGEN Plasmid Midi Kit (25) 3120 12143 25次
QIAGEN Plasmid Midi Kit (100) 11510 12145 100从

 

Qiagen质粒纯化试剂盒QIAGEN Plasmid Midi Kitqiagen 12143

Qiagen质粒纯化试剂盒QIAGEN Plasmid Midi Kit

  • 产品型号:  qiagen 12143
  • 简单描述
  • Qiagen质粒纯化试剂盒纯化Z高产量可达 100 ug的转染级别纯度的质粒或科斯质粒DNA纯度相当于 2倍氯化铯梯度离心高重现性的产量和转染级别纯度经济实惠使用LyseBlue,获得Z佳的裂解和Z大的DNA产量
详细介绍

Qiagen质粒纯化试剂盒QIAGEN Plasmid Midi Kit

传统的QIAGEN Plasmid Giga Kits应用重力流阴离子交换柱QIAGEN-tip 100 高效纯化质粒DNA,也单独提供QIAGEN-tips 100。

QIAGEN Plasmid Midi Kit 特性
预期产量* 柱子容量 培养体积†
Up to 100 µg 100 µg 25 -100 ml

* 实际产量取决于质粒拷贝数、插入片段大小、宿主菌、培养基和培养体积。
† 培养体积取决于质粒拷贝数、插入片段大小、宿主菌和培养基。

原理

QIAGEN *的阴离子交换柱于核酸纯化,其的抽提性能可确保DNA纯度相当于甚至高于两次CsCl梯度离心的产物。预装的QIAGEN柱子以重力流方式纯化,不会流干,将质粒制备所需的手工操作时间降至zui少。整个质粒纯化系统避免了苯酚、氯仿、ethidium bromide和CsCl等有毒物质,将对用户和环境的危害降至zui低。

操作步骤

应用QIAGEN Plasmid Kits纯化过程中,通过离心取出细菌裂解液。然后将澄清的裂解液装入阴离子交换柱,在适当的低盐浓度和pH值条件下,阴离子交换柱会选择性地结合质粒DNA。RNA、蛋白质、代谢物和其他小分子杂质通过中盐浓度洗涤去除,应用高盐浓度缓冲夜洗脱超纯的质粒DNA(见流程图”Comparison of QIAGEN Plasmid Kit Procedures”)。通过异丙醇沉淀浓缩并脱盐DNA,离心法收集DNA。

应用

QIAGEN Plasmid Kits 纯化所得的质粒DNA非常适合于诸如转染、克隆、手动或自动测序(包括毛细管测序)和体外转录等应用。

Images

Qiagen质粒纯化试剂盒QIAGEN Plasmid Midi Kitqiagen 12143 Comparison of QIAGEN Plasmid Kit Procedures Qiagen质粒纯化试剂盒QIAGEN Plasmid Midi Kitqiagen 12143 QIAGEN Plasmid Kit Anion-Exchange Tips Qiagen质粒纯化试剂盒QIAGEN Plasmid Midi Kitqiagen 12143 QIAGEN Plasmid Kits制备超纯的质粒DNA Qiagen质粒纯化试剂盒QIAGEN Plasmid Midi Kitqiagen 12143 转染效率 vs 质粒纯化方法

 

日本同仁化学Hampton蛋白结晶试剂盒3 Well Midi UVP- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > 96 Well Crystallization Plates > Swissci > 3 Well Midi UVP

3 Well Midi UVP

Applications

  • Sitting drop crystallization

Features

  • Lens effect drop wells for enhanced optics
  • Drop volume: 50 nl to 5 µl
  • Micro-numbering alongside drop volumes
  • Reservoir volume: 20 to 40 µl
  • ANSI/SLAS 1-2004 Standard
  • Drop well allows easier crystal harvesting
  • Three drop wells per reservoir
  • Midi profile for easier storage
  • UV compatible (UVP)

Description

The SWISSCI 3 Lens Crystallization Plate is an ideal product for protein crystallization presented in a 96-well plate format.

The plate was developed in collaboration with very experienced protein crystallographers. It is the result of many years of experience in successful robotic high-throughput crystallization and combines many of the necessary features not earlier available to the crystallographer.

The SWISSCI 3 Lens crystallization Plate offers unique properties that make it ideal for both nanoliter crystallization screening and mircoliter optimization alike. Made from optically superior polymer (UVP) and with a new design of the wells, the plate allows easy crystal viewing and retrieval.

The Advantages of the 3 Lens Crystallization Plate Easy crystal retrieval Raised wide wells make the crystal mounting especially easy.

Easy viewing The wells are a wide conical shape and have a lens effect for perfect illumination. The micro-numbering ensures you will never get lost again (visible by microscope). The optically superior polymer is UV transmissible and may be used to differentiate between salt and protein crystals.

Better sealing Wide partition walls between the wells give plenty of area for good sealing with tape. No central bending occurs in this very robust structure. Excellent long term storage – no sample evaporation.

Wide range of volumes Typical volumes are 20-40 µl of reservoir and 50 nl-5 µl drop size. The 288 optical wells offer three times the number of experimental constructions.

ANSI/SLAS 1-2004 Standard The plates are designed to the 96-well ANSI/SLAS 1-2004 standards for all common holders and external numbering (A-H, 1-12)with corner location make the plate easy to use in a robotic sampler. The 3 Lens plate is suitable for centrifugation. The unique 3 drop protein crystallization plate offers a new way of sitting-drop crystallography. The 288 wells are optically perfect designed to observe crystals under a microscope.

3 Lens plate features Easy to fill 96-lens structure – typically 30 microlitres per buffer lens. Standard ANSI/SLAS 1-2004 form for automation. Optically perfect wells with lens effect for a better view with microscope. Microscopic identifier within wells, simplifying the orientation under the microscope. Maximum volume of the buffer reservoir is 40 microlitres. Grown crystals are easy to identify and to remove from lens due to a low-binding polymer. Plate with 3 lenses for each sample, better growing security with triplicates or the ability to use lens two and lens three as mixing stations. Lenses fill without micro-droplets jumping out due to static effects. Wells can be individually sealed with a perfectly flat upper surface – there is a large land area designed to ensure integrity of each lens section. The profile allows for easier storage. Low volume buffer well enables savings on reagents. Triplicates allow for 288 constructs per plate. 

Show More

Hampton蛋白结晶试剂盒3 Well Midi UVP

Click to Zoom In

Hampton蛋白结晶试剂盒3 Well Midi UVP
Hampton蛋白结晶试剂盒3 Well Midi UVP
Hampton蛋白结晶试剂盒3 Well Midi UVP
Hampton蛋白结晶试剂盒3 Well Midi UVP
Hampton蛋白结晶试剂盒3 Well Midi UVP
Hampton蛋白结晶试剂盒3 Well Midi UVP

CAT NO

HR3-123

NAME

Swissci 3 Well Midi Crystallization Plate in UVP (10)

DESCRIPTION

10 plate case

PRICE

$102.00

cart quote

CAT NO

HR3-125

NAME

Swissci 3 Well Midi Crystallization Plate in UVP (40)

DESCRIPTION

40 plate case

PRICE

$394.00

cart quote

Support Material(s)

Hampton蛋白结晶试剂盒3 Well Midi UVP Swissci 3 Well Midi (HR3-123/HR3-125) Plate Drawing

Related Item(S)

  • ClearSeal Film™ and Applicator
  • Crystal Clear Sealing Tape
  • Crystal Clear Sealing Film

日本同仁化学Hampton蛋白结晶试剂盒3 Well Midi UVXPO- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > 96 Well Crystallization Plates > Swissci > 3 Well Midi UVXPO

3 Well Midi UVXPO

Applications

  • Sitting drop crystallization

Features

  • Lens effect drop wells for enhanced optics
  • Drop volume: 50 nl to 5 µl
  • Micro-numbering alongside drop volumes
  • Reservoir volume: 20 to 40 µl
  • ANSI/SLAS 1-2004 Standard
  • Drop well allows easier crystal harvesting
  • Three drop wells per reservoir
  • Midi profile for easier storage
  • UV compatible

Description

The SWISSCI 3 Lens Crystallization Plate is an ideal product for protein crystallization presented in a 96-well plate format.

The SWISSCI UVXPO 3 Lens Crystallisation Plate is made from a new polymer mixture that means crystals can be viewed in UV, Polar and Visible light. UVXPO is short for UV compatible as well as excellent uniform (zero) background when using cross polarised light.

The SWISSCI 3 Lens Crystallisation Plate is an ideal product for protein crystallisation presented in a 96-well plate format.

The Advantages of the 3 Lens Crystallization Plate

Easy crystal retrieval
Raised wide wells make the crystal mounting especially easy.

Easy viewing
Crystals can be viewed in UV, Polar and Visible light.
Uniform (zero) background when using cross polarised light.
The wells are a wide conical shape and have a lens effect for perfect illumination.
The micro-numbering ensures you will never get lost again (visible by microscope).
The optically superior polymer is UV transmissible and may be used to differentiate between salt and protein crystals.

Better sealing
Wide partition walls between the wells give plenty of area for good sealing with tape.
No central bending occurs in this very robust structure.
Excellent long term storage – no sample evaporation.

Wide range of volumes
Typical volumes are 20-40 µl of reservoir and 50 nl-5 µl drop size. The 288 optical wells offer three times the number of experimental constructions.

ANSI/SLAS 1-2004 Standard
The plates are designed to the 96-well ANSI/SLAS 1-2004 standard for all common holders and external numbering (A-H, 1-12) with corner location make the plate easy to use in a robotic sampler.
The 3 Lens plate is suitable for centrifugation.
The unique 3 drop protein crystallisation plate offers a new way of sitting-drop crystallography.
The 288 wells are optically perfect designed to observe crystals under a microscope.

3 Lens plate features 
Easy to fill 96-lens structure – typically 30 microlitres per buffer lens.
Standard ANSI/SLAS 1-2004 Standard form for automation.
Optically perfect wells with lens effect for a better view with microscope.
Microscopic identifier within wells, simplifying the orientation under the microscope.
Maximum volume of the buffer reservoir is 40 microlitres, typically 10 nanolitres is used for the protein well filling.
Grown crystals are easy to identify and to remove from lens due to a low-binding polymer.
Plate with 3 lenses for each sample, better growing security with triplicates or the ability to use lens two and lens threeas mixing stations.
Lenses fill without micro-droplets jumping out due to static effects.
Wells can be individually sealed with a perfectly flat upper surface – there is a large land area designed to ensureintegrity of each lens section.
The profile allows for easier storage.
Low volume buffer well enables savings on reagents.
Triplicates allow for 288 constructs per plate.

Show More

Hampton蛋白结晶试剂盒3 Well Midi UVXPO

Click to Zoom In

Hampton蛋白结晶试剂盒3 Well Midi UVXPO
Hampton蛋白结晶试剂盒3 Well Midi UVXPO
Hampton蛋白结晶试剂盒3 Well Midi UVXPO
Hampton蛋白结晶试剂盒3 Well Midi UVXPO
Hampton蛋白结晶试剂盒3 Well Midi UVXPO
Hampton蛋白结晶试剂盒3 Well Midi UVXPO

CAT NO

HR3-193

NAME

Swissci 3 Well Midi Crystallization Plate in UVXPO (10)

DESCRIPTION

10 plate case

PRICE

$102.00

cart quote

CAT NO

HR3-194

NAME

Swissci 3 Well Midi Crystallization Plate in UVXPO (40)

DESCRIPTION

40 plate case

PRICE

$394.00

cart quote

Support Material(s)

Hampton蛋白结晶试剂盒3 Well Midi UVXPO Swissci 3 Well Midi UVXPO (HR3-193/HR3-194) Plate Drawing

Related Item(S)

  • ClearSeal Film™ and Applicator
  • Crystal Clear Sealing Tape
  • Crystal Clear Sealing Film

References

 

德国IKA Midi MR1 digital数显型磁力搅拌器IKA Midi MR 1

德国IKA Midi MR1 digital数显型磁力搅拌器

简要描述:德国IKA Midi MR1 digital数显型磁力搅拌器强功率磁力搅拌器,无加热功能。搅拌量多达 50 升 (H2O)平整、坚固的不锈钢外壳电子马达无级变速

产品型号: IKA Midi MR 1

所属分类:磁力搅拌器

详细说明:
品牌 IKA/德国艾卡 产地类别 进口
价格区间 1万-5万 仪器种类 磁力搅拌器
应用领域 医疗卫生,环保,化工,生物产业

产品特点:

德国IKA Midi MR1 digital数显型磁力搅拌器

强功率磁力搅拌器,无加热功能。

搅拌量多达 50 升 (H2O)

平整、坚固的不锈钢外壳

电子马达

无级变速

数字式 LED 速度显示屏

定时(0 – 56 分钟)或连续运行

集成式 USB 和 RS 232 接口

德国IKA Midi MR1 digital数显型磁力搅拌器

强功率磁力搅拌器,无加热功能。

搅拌量多达 50 升 (H2O)

平整、坚固的不锈钢外壳

电子马达

无级变速

技术参数:

搅拌点位数目

1

大搅拌量 (H2O)

50 l

电机输出功率

19 W

速度范围

0 – 1000 rpm

搅拌子大长度

80 mm

加热盘自热(室温:22°C/保持:1小时)

28 +K

工作盘材质

不锈钢 1.4301

工作盘外形尺寸

350 x 350 mm

外形尺寸

360 x 110 x 430 mm

重量

10.7 kg

允许环境温度

5 – 40 °C

允许相对湿度

80 %

DIN EN 60529 保护方式

IP 21

RS 232接口

USB接口

电压

230 / 100 – 120 / 100 V

频率

50/60 Hz

仪器输入功率

70 W