The SK7 (Leu-4) monoclonal antibody specifically binds to the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa). The CD3 antigen is present on 61% to 85% of normal peripheral blood lymphocytes 60% to 85% of thymocytes and on Purkinje cells in cerebellum. The soluble form of this antibody has a mitogenic effect on most peripheral blood T lymphocytes, provided appropriate functional monocytes are present.
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
557851
FORMAT
Format
PE-Cy™7
Excitation Source
Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
Excitation Max
496 nm
Emission Max
785 nm
PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.
The RPA-T4 monoclonal antibody specifically binds to CD4, a 59 kDa single-chain transmembrane glycoprotein that is expressed on T-helper/inducer cell populations. CD4 is also expressed on thymocytes subsets and at lower levels on monocytes and macrophages. CD4 functions as a co-receptor in MHC class II-restricted antigen-induced T cell activation and as a receptor for human immunodeficiency viruses (HIV). This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and reacts with approximay 80% of thymocytes and 45% of peripheral blood lymphocytes. RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development or are reported in the literature.
FORMAT
Format
PE
Excitation Source
Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
Excitation Max
496 nm
Emission Max
578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Other Formats →
APC
APC-Cy™7
APC-H7
APC-R700
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 700
BB515
BUV395
BV421
BV605
Biotin
FITC
NA/LE
PE-CF594
PE-Cy™5
PE-Cy™7
Pacific Blue™
PerCP-Cy™5.5
Purified
SUGGESTED COMPANION PRODUCTS
Cat No.
Description
Size
555749
PE Mouse IgG1, κ Isotype Control RUO
100 Tests
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。ｃｏｍ/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
BD human CD8抗体 Clone RPA-T8宿主物种：mouse反应物种：human应用：流式现货供应
详细介绍
BD human CD8抗体 Clone RPA-T8
Alternative Name：CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
BD human CD8抗体 Clone RPA-T8
Reactivity
Human (QC Testing) Rhesus, Cynomolgus, Baboon (Tested in Development)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human CD8a
Workshop No.
IV T171; V T-CD08.03; VI 6T-CD8.1, 6T-081
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
DESCRIPTION
The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α) . CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when disulfide-bonded to a CD8 beta chain (CD8β) . CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8 α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors.
FORMAT
Format
FITC
Excitation Source
Blue 488 nm
Excitation Max
494 nm
Emission Max
520 nm
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Other Formats →
APC
APC-Cy™7
APC-R700
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 700
BB515
BUV395
BUV496
BUV563
BV421
BV510
BV650
BV711
BV786
Biotin
NA/LE
PE
PE-CF594
PE-Cy™5
SUGGESTED COMPANION PRODUCTS
Cat No.
Description
Size
555748
FITC Mouse IgG1, κ Isotype Control RUO
100 Tests
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。ｃｏｍ/colors.
Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
