sigma MTTpowder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC）
详细介绍
sigma MTT
Product Specification Product Number: M5655 CAS Number: 298-93-1 MDL: MFCD00011964 Formula: C18H16BrN5S Formula Weight: 414.32 g/mol Storage Temperature: 2 – 8 °C T_E_S_T________________________________________Sp_e_c_if_ic_at_io_n______________________ Appearance （Color） Yellow to Dark Yellow Appearance （Form） Powder Infrared spectrum Conforms to Structure Insect Cell Test Pass Performance Pass Purity （HPLC） >_ 97.5 % Solubility （Turbidity） Clear c = 5mg/ml; Water Solubility （Color） Yellow to Very Dark Yellow and Green-Yellow to Very Dark Green-Yellow and Yellow-Green Recommended Retest Period ————————- 5 Years
sigma脱氧核糖核酸酶I Dnase IType IV, lyophilized powder, ≥2,000 Kunitz units/mg protein
详细介绍
sigma脱氧核糖核酸酶I Dnase I
Quality Level
PREMIUM
type
Type IV
form
lyophilized powder
mol wt
mol wt ~31 kDa
purified by
chromatography
foreign activity
Chymotrypsin ≤0.5%
Protease ≤0.05%
sigma脱氧核糖核酸酶I Dnase I
Product Specification Product Number: D5025 CAS Number: 9003-98-9 MDL: MFCD00130918 Storage Temperature: -20 °C T_E_S_T________________________________________Sp_e_c_if_ic_at_io_n______________________ Appearance （Color） White to Light Tan Appearance （Form） Powder Solubility （Color） Colorless Solubility （Turbidity） Clear 5 mg/ml, 0.15 M NaCl % Protein （Biuret） >_ 80 Kunitz units/mg protein >_ 2000 One Kunitz unit will produce a change in A260 of 0.001 per minute per mL at pH 5.0 at 25 Deg C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM. Chymotrypsin <_ 0.5 % Protease <_ 0.05 % % RNAse <_ 0.02 % Recommended Retest Period ————————- 3 years Specification: PRD.1.ZQ5.10000007039 Sigma-
T_E_S_T________________________________________Sp_e_c_if_ic_at_io_n______________________ Appearance （Color） Colorless Appearance （Form） Liquid Infrared spectrum Conforms to Structure Color Test <_ 10 APHA UV Absorbance 400nm <_ 0.01 UV Absorbance 260nm <_ 0.01 UV Absorbance 240nm <_ 0.05 UV Absorbance 230nm <_ 0.15 UV Absorbance 220nm <_ 0.30 UV Absorbance 210nm <_ 0.60 UV Absorbance 203nm <_ 1.0 Purity （GC） >_ 99.925 % Water （by Karl Fischer） <_ 0.02 % Residue on Evaporation <_ 0.0003 % Suitability Suitable Suitable for Use as a Protein Sequencing Reagent Titrable Acid （meq/g） <_ 0.0003 Titrable Base （meq/g） <_ 0.0002 Specification: PRD.2.ZQ5.10000005439 Sigma-Aldrich warrants, that at the time of the quality release or subsequent retest date this product conformed to the information contained in this publication. The current Specification sheet may be available at Sigma-Aldrich.com. For further inquiries, please contact Technical Service. Purchaser must determine the suitability of the product for its particular use. See reverse side of invoice or packing slip for additional terms and conditions of sale
FREUND'S ADJUVANT, COMPLETE AND INCOMPLETE Product Number F 5881 AND F 5506 Storage Temperature 2-8 °C Product Description Appearance F 5881 Clear amber liquid containing particulate matter （dried cells） F 5506 Clear amber liquid Freund's Adjuvant is one of the most commonly used adjuvants in research. It is used as a water-in-oil emulsion. It is prepared from non-metabolizable oils （paraffin oil and mannide monooleate）. If it also contains killed Mycobacterium tuberculosis it is known as Complete Freund's Adjuvant. Without the bacteria it is Incomplete Freund's Adjuvant. First developed by Jules Freund in the 1940's, Freund's Adjuvant is designed to provide continuous release of antigens necessary for stimulating a strong, persistent immune response1,2,3 The main disadvantage of Freund's Adjuvant is that it can cause granulomas, inflammation at the inoculation site and lesions. The mycobacteria in Complete Freund's attracts macrophages and other cells to the injection site which enhances the immune response. For this reason, the Complete Freund's Adjuvant is used for the initial injections. To minimize side-effects, Incomplete Freund's Adjuvant is used for the boosts. For comparisons of different adjuvant systems, see references 4 and 5. Reagents Each ml of F 5881 contains 1 mg of heat-killed and dried Mycobacterium tuberculosis （strain H37Ra, ATTC 25177）, 0.85 ml paraffin oil and 0.15 ml of mannide monooleate. Each ml of F 5506 contains 0.85 ml of paraffin oil and 0.15 ml of mannide monooleate. Precautions and Disclaimer Please consult the Material Safety Data Sheet for handling recommendations before working with this material. Storage/Stability Store in a cooler at 2-8 °C. Do not Freeze. Procedure 1. If using Complete Freund’s Adjuvant, vortex or shake to resuspend the Mycobacterium. 2. Mix antigens （preferably in saline） with an equal volume of the adjuvant to form an emulsion. In order to do this, vigorous and prolonged mixing is needed. There are at least three methods which can be used to accomplish this: For small volumes the emulsion can be made in a tube. Pipet the adjuvant in the tube first. Then, while vortexing, add an equal volume of the antigen solution. Vortex vigorously until a thick emulsion forms. For intermediate volumes, use two syringes connected through a luer fitting. Ideally, a 3-way valve should be used. Take the desired amount of antigen solution into a glass syringe. The volume should not fill more than half the syringe. Take an equal volume of the adjuvant into another glass syringe. Remove all air and connect the syringes through the luer fitting to the 3-way valve. Adjust the 3-way valve such that the connection is open between the two syringes. Carefully depress the plunger from the antigen solution first, pushing the antigen into the oil of the adjuvant. Alternay push the plungers, mixing the adjuvant and the antigen solution into an emulsion. Continue until the plungers are difficult to push. For large volumes, use a tissue homogenizer to make the emulsion. Add the adjuvant to the homogenizer first. Run the homogenizer for a short time to coat the inside with the adjuvant. Add an equal volume of the antigen solution and run until a thick emulsion forms. 3. The resulting emulsion should be very thick and a drop of it should not disperse if tested by placing on the surface of a saline solution. 4. Transfer the emulsion to a syringe （or remove one syringe from the luer fitting if using the two-syringe method）. Remove all the air. Add an appropriay sized needle. The samples are now ready for injection.6 sigma弗氏不*佐剂
References 1. Freund, J. and McDermott, K., Proc. Soc. Exp. Biol. Med., 49, 548-553 （1942） 2. Freund, J., Ann. Rev. Microbiol., 1, 291 （1947） 3. Freund, J., Adv. Tuberc. Res., 7, 130 （1956） 4. Bennett, B. et al., J. Immuno. Meth., 153, 31-40 （1992） 5. Deeb, B.J. et al., J. Immuno. Meth., 152, 105-113 （1992） 6. Harlow, E. and Lane, D., Antibodies A Laboratory Manual, （Cold Spring Harbor Laboratory, 1988）
sigma白蛋白Albumin鸡卵清蛋白type Grade II from chicken egg white assay 62-88% (agarose gel electrophoresis) form powder mol wt mol wt 44287 Da by calculation storage temp. 2-8°
sigma赖氨酸-CEndoproteinase Lys-Cfrom Lysobacter enzymogenessuitable for protein sequencing, lyophilized powder packaging vial of 5 µg suitability suitable for protein sequencing storag
详细介绍
sigma赖氨酸-CEndoproteinase Lys-C
Product Name
Endoproteinase Lys-C from Lysobacter enzymogenes, suitable for protein sequencing, lyophilized powder
are available for this Product.