检查场所

拭取方法

钳子管道开口

尽量将棉棒伸到可以拭取的部位，边转动棉棒边仔细拭取

吸引管道接口

通气、送水管道开口

先端部

镜头与前端部的外周1cm范围

钳子管道内部（使用LuciSwab）

从钳子管道口插入进行拭取
     从先端部插入进行拭取

组成品

状态

包装

OVA包被96孔板（干燥板）

清洗后使用

96 wells（8×12）／1个

标准溶液（Anti OVA- IgG1:1,200U/mL）（单抗）

稀释后使用

100 μL/1瓶

缓冲液

直接使用

60 mL/1瓶

生物素结合抗小鼠IgG1抗体（单抗）

稀释后使用

200 μL/1瓶

过氧化物酶·抗生素结合物

稀释后使用

200 μL/1瓶

显色液（TMB）

直接使用

12 mL/1瓶

终止液（1M H₂SO₄）

※小心轻放

直接使用

12 mL/1瓶

浓缩清洗液（10×）

稀释后使用

100 mL/1瓶

孔板密封膜

3个

产品说明书

1本

样本

A

B

1

105

18.2

2

100

16.9

3

96.2

16.7

4

100

17.4

5

106

17.4

Mean

101

17.3

SD

4.0

0.59

CV（%）

4.0

3.4

测定日/样本

C

D

E

0天

60.1

15.0

3.75

1天

58.5

14.7

3.70

2天

59.9

15.3

3.83

3天

61.4

15.7

3.82

Mean

60.0

15.2

3.77

SD

1.2

0.44

0.06

CV（%）

2.0

2.9

1.6

添加量

实测值

回收量

回收率（%）

0.00

8.86

—

—

7.29

15.8

6.94

95.2

11.0

19.5

10.6

96.4

14.6

23.0

14.1

96.6

添加量

实测值

回收量

回收率（%）

0.00

51.5

—

—

26.9

76.8

25.3

97.1

31.4

80.6

29.1

92.5

47.1

100

48.5

103

 1.

Pinocembrin attenuates allergic airway inflammation via inhibition of NF-κB pathway in mice. Gu X, Zhang Q, Du Q, Shen H, Zhu Z. Int Immunopharmacol. 2017 Oct 18;53:90-95.

 2.

A dichloromethane fraction of Triticum aestivum sprouts reduces allergic immune response through inhibiting Th2 differentiation in ovalbumin‑immunized mice. Ki HH, Hwang SW, Lee JH, Kim YH, Kim DK, Lee YM. Mol Med Rep. 2017 Sep;16(3):3535-3541.

 3.

Urban PM2.5 exacerbates allergic inflammation in the murine lung via a TLR2/TLR4/MyD88-signaling pathway. He M, Ichinose T, Yoshida Y, Arashidani K, Yoshida S, Takano H, Sun G, Shibamoto T. Sci Rep. 2017 Sep 8;7(1):11027.

 4.

Activation of group 2 innate lymphoid cells exacerbates and confers corticosteroid resistance to mouse nasal type 2 inflammation. Morikawa T, Fukuoka A, Matsushita K, Yasuda K, Iwasaki N, Akasaki S, Fujieda S, Yoshimoto T. Int Immunol. 2017 May 1;29(5):221-233.

 5.

Aquaporin-3 potentiates allergic airway inflammation in ovalbumin-induced murine asthma. Ikezoe K, Oga T, Honda T, Hara-Chikuma M, Ma X, Tsuruyama T, Uno K, Fuchikami J, Tanizawa K, Handa T, Taguchi Y, Verkman AS, Narumiya S, Mishima M, Chin K. Sci Rep. 2016 May 11;6:25781.

 6.

Exposure to bisphenol A enhanced lung eosinophilia in adult male mice. He M, Ichinose T, Yoshida S, Takano H, Nishikawa M, Shibamoto T, Sun G. Allergy Asthma Clin Immunol. 2016 Apr 14;12:16.

 7.

Differences in allergic inflammatory responses between urban PM2.5 and fine particle derived from desert-dust in murine lungs. He M, Ichinose T, Kobayashi M, Arashidani K, Yoshida S, Nishikawa M, Takano H, Sun G, Shibamoto T. Toxicol Appl Pharmacol. 2016 Apr 15;297:41-55.

 8.

Desert dust induces TLR signaling to trigger Th2-dominant lung allergic inflammation via a MyD88-dependent signaling pathway. He M, Ichinose T, Song Y, Yoshida Y, Bekki K, Arashidani K, Yoshida S, Nishikawa M, Takano H, Shibamoto T, Sun G. Toxicol Appl Pharmacol. 2016 Apr 1;296:61-72.

 9.

Administration of Pigment Epithelium-Derived Factor Inhibits Airway Inflammation and Remodeling in Chronic OVA-Induced Mice via VEGF Suppression. Zha W, Su M, Huang M, Cai J, Du Q. Allergy Asthma Immunol Res. 2016 Mar;8(2):161-9.

10.

Low-dose benzo[a]pyrene aggravates allergic airway inflammation in mice. Yanagisawa R, Koike E, Win-Shwe TT, Ichinose T, Takano H. J Appl Toxicol. 2016 Feb 25.

11.

Prevention of allergic rhinitis by ginger and the molecular basis of immunosuppression by 6-gingerol through T cell inactivation. Kawamoto Y, Ueno Y, Nakahashi E, Obayashi M, Sugihara K, Qiao S, Iida M, Kumasaka MY, Yajima I, Goto Y, Ohgami N, Kato M, Takeda K. J Nutr Biochem. 2016 Jan;27:112-22.

12.

Immunotoxic Effect of Low-Dose Methylmercury Is Negligible in Mouse Models of Ovalbumin or Mite-Induced Th2 Allergy. Nakamura R, Takanezawa Y, Sone Y, Uraguchi S, Sakabe K, Kiyono M.

Biol Pharm Bull. 2016;39(8):1353-8.

13.

Prevention of allergic rhinitis by ginger and the molecular basis of immunosuppression by 6-gingerol through T cell inactivation. Yoshiyuki Kawamoto, Yuki Ueno, Emiko Nakahashi, Momoko Obayashi, Kento Sugihara, Shanlou Qiao, Machiko Iida, Mayuko Y. Kumasaka, Ichiro Yajima, Yuji Goto, Nobutaka Ohgami, Masashi Kato, Kozue Takeda. The Journal of Nutritional Biochemistry, Volume 27, Jan. 2016, Pages 112–122

14.

Effects of Sohamhyoong-Tang on Ovalbumin-Induced Allergic Reaction in BALB/c Mice. Jo SH, Lee YJ, Kang DG, Lee HS, Kim DK, Park MC. Evid Based Complement Alternat Med. 2016;2016:6286020.

15.

Effect of diosmetin on airway remodeling in a murine model of chronic asthma. Ge A, Liu Y, Zeng X, Kong H, Ma Y, Zhang J, Bai F, Huang M. Acta Biochim Biophys Sin (Shanghai). Vol.47(8), p604-11, Aug 2015.

16.

PM2.5-rich dust collected from the air in Fukuoka, Kyushu, Japan, can exacerbate murine lung eosinophilia. He M, Ichinose T, Ren Y, Song Y, Yoshida Y, Arashidani K, Yoshida S, Nishikawa M, Takano H, Sun G. Inhal Toxicol. Vol.27(6), p287-99, May 2015.

17.

Anti-asthma potential of crocin and its effect on MAPK signaling pathway in a murine model of allergic airway disease. Xiong Y, Wang J, Yu H, Zhang X, Miao C. Immunopharmacol Immunotoxicol. Vol.10, p1-8, Mar 2015.

18.

Roles of lipoxin A4 receptor activation and anti-interleukin-1β antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-κB pathway in airway inflammation induced by ovalbumin. Kong X, Wu SH, Zhang L, Chen XQ. Mol Med Rep. 2015 Mar 5

19.

Pharyngeal aspiration of metal oxide nanoparticles showed potential of allergy aggravation effect to inhaled ovalbumin. Horie M, Stowe M, Tabei M, Kuroda E. Inhal Toxicol. Vol.27(3), p181-90, Feb 2015.

20.

Oxidized dietary oils enhance immediate- and/or delayed-type allergic reactions in BALB/c mice. Ogino H, Sakazaki F, Okuno T, Arakawa T, Ueno H. Allergol Int. Vol.64(1), p66-72, Jan 2015.

21.

The effects of nodakenin on airway inflammation, hyper-responsiveness and remodeling in a murine model of allergic asthma. Xiong Y, Wang J, Yu H, Zhang X, Miao C, Ma S. Immunopharmacol Immunotoxicol. Vol.36(5), p341-348, Oct 2014.

22.

Allogeneic pluripotent stem cells suppress airway inflammation in murine model of acute asthma. Ogulur I, Gurhan G, Kombak FE, Filinte D, Barlan I, Akkoc T. International Immunopharmacology, Vol.22(1), p31-40 Sep 2014.

23.

Effects of prior oral exposure to combinations of environmental immunosuppressive agents on ovalbumin allergen-induced allergic airway inflammation in Balb/c mice. Fukuyama T, Nishino R, Kosaka T, Watanabe Y, Kurosawa Y, Ueda H, Harada T.. Immunopharmacol Immunotoxicol. Vol.36(4), p261-70, Aug 2014.

24.

Enhancement of OVA-induced murine lung eosinophilia by co-exposure to contamination levels of LPS in Asian sand dust and heated dust. Ren Y, Ichinose T, He M, Song Y, Yoshida Y, Yoshida S, Nishikawa M, Takano H, Sun G, Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.10(1), Jun 2014.

25.

A bacterial extract of OM-85 Broncho-Vaxom prevents allergic rhinitis in mice. Han L, Zheng CP, Sun YQ, Xu G, Wen W, Fu QL. American Journal of Rhinology & Allergy, Vol.28(2), p110-116, Mar-Apr 2014.

26.

Broncho-Vaxom Attenuates Allergic Airway Inflammation by Restoring GSK3β-Related T Regulatory Cell Insufficiency. Fu R, Li J, Zhong H, Yu D, Zeng X, Deng M, Sun Y, Wen W, Li H. PLoS One. 2014 Mar 25;9(3):e92912

27.

Lung inflammation by fungus, Bjerkandera adusta isolated from Asian sand dust (ASD) aerosol and enhancement of ovalbumin-induced lung eosinophilia by ASD and the fungus in mice. Liu B, Ichinose T, He M, Kobayashi F, Maki T, Yoshida S, Yoshida Y, Arashidani K, Takano H, Nishikawa M, Sun G, Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.10(1), Feb 2014.

28.

Midazolam inhibits IgE production in mice via suppression of class switch recombination. Kusama H, Kobayashi R, Kurita-Ochiai T. Journal of Oral Science, Vol.56(1), p77-83, 2014.

29.

Induction of immune tolerance and reduction of aggravated lung eosinophilia by co-exposure to Asian sand dust and ovalbumin for 14 weeks in mice. He M., Ichinose T., Yoshida S., Takano H., Nishikawa M., Sun G. and Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.9(19), 2013.

30.

Galangin Abrogates Ovalbumin-Induced Airway Inflammation via Negative Regulation of NF-B. Zha W-J., Qian Y., Shen Y., Du Q., Chen F-F., Wu Z-Z., Li X. and Huang M. Evidence-Based Complementary and Alternative Medicine, Vol.2013 (2013), p14.

31.

Effects of two Asian sand dusts transported from the dust source regions of Inner Mongolia and northeast China on murine lung eosinophilia. M.He, T.Ichinose, Y.Song, Y.Yoshida, K.Arashidani, S.Yoshida, B.Liu, M.Nishikawa, H.Takano, G.Sun. Toxicology and Applied Pharmacology, Available online 26 July 2013.

32.

Effect of the size of receptor in allergy detection using field effect transistor biosensor. S.Hideshima, S.Kuroiwa, M.Kimura, S.Cheng, T.Osaka. Electrochimica Acta, Available online 24 July 2013.

33.

Elevated Macrophage Inflammatory Protein 1α and Interleukin-17 Production in an Experimental Asthma Model Infected with Respiratory Syncytial Virus. T.Ishioka, Y.Yamada, H.Kimura, M.Yoshizumi, H.Tsukagoshi, K.Kozawa, K.Maruyama, Y.Hayashi, M.Kato. Int Arch Allergy Immunol, Vol.161(suppl 2), p129-137, May 2013.

34.

Leukotriene B4 receptor BLT2 negatively regulates allergic airway eosinophilia . Y.Matsunaga, S.Fukuyama, T.Okuno, F.Sasaki, T. Matsunobu, Y.Asai, K.Matsumoto, K.Saeki, M.Oike, Y.Sadamura, K.Machida, Y.Nakanishi, M.Kubo, T.Yokomizo and H.Inoue. The FASEB Journal, Published online before print April 19, 2013.

35.

Effects of exposure to nanoparticle-rich or -depleted diesel exhaust on allergic pathophysiology in the murine lung. Tanaka M., Aoki Y., Takano H., Fujitani Y., Hirano S., Nakamura R., Sone Y., Kiyono M., Ichinose T., Itoh T., Inoue K. Journal of Toxicological Sciences, Vol.38(1), p35-48, Feb 2013.

36.

HIF-1α Inhibition Reduces Nasal Inflammation in a Murine Allergic Rhinitis Model. Zhou H, Chen X, Zhang W-M, Zhu L-P, Cheng L. PLOS one, 2012.

37.

Human Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Allergic Airway Inflammation in Mice. Sun Y-Q, Deng M-X, He J, Zeng Q-X, Wen W, Wong D S.H, Tse H-F, Xu G, Lian Q, Shi J, Fu Q-L. STEM CELLS, Vol.30(12), p2692-2699, Dec 2012.

38.

Aggravating effects of Asian sand dust on lung eosinophilia in mice immunized beforehand by ovalbumin. He M, Ichinose T, Yoshida S, Takano H, Nishikawa M, Mori I, Sun G, Shibamoto T. Inhalation Toxicology, Vol.24(11) , p751-761, Sep 2012.

39.

Attenuation of airway hyperreactivity and T helper cell type 2 responses by coumarins from Peucedanum praeruptorum Dunn in a murine model of allergic airway inflammation. Xiong Y-Y, Wu F-H, Wang J-S, Li J, Kong L-Y. Journal of Ethnopharmacology, Vol.141(1), p314-321, May 2012.

40.

Effects of lysed Enterococcus faecalis FK-23 on experimental allergic rhinitis in a murine model. Zhu L,Shimada T, Chen R, Lu M, Zhang Q, Lu W, Yin M, Enomoto T, Cheng L. Journal of Biomedical Research, Vol.26(3), p226-234, May 2012.

41.

Sphingosine-kinase 1 and 2 contribute to oral sensitization and effector phase in a mouse model of food allergy. S. C. Diesner., A. Olivera., S. Dillahunt., C. Schultz., T. Watzlawek., E. Forster-Waldl., A. Pollak., E. Jensen-Jarolim., E. Untersmayr., J. Rivera. Immunology Letters, Vol. 141, Issue 2, 30 January 2012, Pages 210-219

42.

Identification of Semaphorin 4B as a Negative Regulator of Basophil-Mediated Immune Responses. Y. Nakagawa., H. Takamatsu., T. Okuno., S. Kang., S. Nojima., T. Kimura., T. R. Kataoka., M. Ikawa., T. Toyofuku., I. Katayama., and A. Kumanogoh. The Journal of Immunology, March 1, 2011 vol. 186 no. 5 2881-2888

43.

Suppression of ovalbumin-induced allergic diarrhea by diminished intestinal peristalsis in RAMP1-deficient mice. R. Yoshikawa., N. Mikami., I. Otani., T. Kishimoto., S. Nishioka., N. Hashimoto., Y. Miyagi., Y. Takuma., K. Sueda., S. Fukada., H. Yamamoto., K. Tsujikawa. Biochemical and Biophysical Research Communications, Vol. 410, Issue 3, 8 July 2011, Pages 389-393

44.

Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4+CD25+Foxp3+ regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model. H.-J. Kim., H. J. Lee., S.-J. Jeong., H.-J. Lee., S.-H. Kim., E.-J. Park. Journal of Ethnopharmacology, Vol. 138, Issue 1, 31 October 2011, Pages 40-46

45.

Urban particulate matter in Beijin,China,enhances allergen-induced murine lung eosinophilia. He,M.,Ichinose,T.,Yoshida,S.,Nishikawa,M.,Mori,I.,Yanagisawa,R.,Takano,H.,Inoue,K.,Sun,G.,Shibamoto,T. Inhalation Toxicology 22(9):709-718,August,2010

46.

Deficiency in the Serum-Derived Hyaluronan-Associated Protein-Hyaluronan Complex Enhances Airway Hyperresponsiveness in a Murine Model of Asthma. L. Zhu., L. Zhuo., K. Kimata., E. Yamaguchi., H. Watanabe., M. A. Aronica., V. C. Hascall., K. Baba. Int Arch Allergy Immunol 153:223-233 2010

47.

Thioredoxin suppresses airway inflammation independently of systemic Th1/Th2 immune modulation. M. Torii., L. Wang., N. Ma., K. Saito., T. Hori., M. Sato-Ueshima., Y. Koyama., H. Nishikawa., N. Katayama., A. Mizoguchi., H. Shiku., J. Yodoi., K. Kuribayashi., T. Kato. European Journal of Immunology Vol.40(3) 787-796 2010

48.

Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Yanase,Y.,Hiragun,T.,Uchida,K.,Ishii,K.,Oomizu,S.,Suzuki,H.,Mihara,S.,Iwamoto,K.,Matsuo,H.,Onishi,N.,Kameyoshi,Y.,and Hide,M. Biochemical and Biophysical Research Communications 387:3:435- 439,2009

49.

Peroxisome Proliferator-Activated Receptor gNegatively Regulates Allergic Rhinitis in Mice. Fukui,N.,Honda,K.,Ito,E.,and Ishikawa K. Allergology Internatioal.58:247-253,2009

50.

IL-16 Variabillity and Modulation by Antiallergic Drugs in a Murine Experimental Allergic Rhinitis Model. Akiyama,K.,Karaki,M.,Kobayshi,R.,Dobashi,H.,Ishida,T, and Mori,N. Allergy and Immunology 149:4,2009

51.

Frequency of Foxp3+CD4+CD25+ T cell is associated with the phenotypes of allergic asthma. Matsumoto,K.,Inoue,H.,Fukuyama,A.,Kan,O,K.,Eguchi,T,M.,Matsumoto,T.,Moriwaki,A.,Nakano,T., and Nakanishi,Y. Respirology 14:2,2009

52.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T Cells in Th2-Type Airway Inflammation. Saito, K., Torii, M., Ning Ma, Tsuchiya, T., Wang, L., Hori, T., Nagakubo, D., Nitta, N.,Kanegasaki, S., Hieshima, K., Yoshie, O., Gabazza, E.C., Katayama, N., Shiku, H.,Kuribayashi, K. and Kato, T. The Journal of Immunology, 181:6889-6897, 2008

53.

Effects of Asian Sand Dust, Arizona Sand Dust, Amorphous Silica and Aluminum Oxide on Allergic Inflammation in the Murine Lung. Ichinose, T., Yoshida, S., Sadakane, K., Takano, H., Yanagizawa, R., Inoue, K., Nishikawa, M.,Mori, I., Kawazato, H., Yasuda, A. and Shibamoto, T. Inhalation Toxicology, Volume 20, Issue 7, 685-694, 2008

54.

The Effects of Microbial Materials Adhered to Asian Sand Dust on Allergic Lung Inflammation. Ichinose,T., Yoshida,S., Hiyoshi,K., Sadakane,K., Takano,H., Nishikawa,M., Mori,I., Yanagisawa,R., Kawazato,H., Yasuda,A., and Shibamoto,T. Arch Environ Contam Toxicol 55:348-357,2008

55.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T cell in Th2-Type Airway Inflammation. Saito,K., Torii,M., Ma,N., Tsuchiya,T., Wang,L.,Hori,T., Nagakubo,D., Nitta,N., Kanegasaki,S., Hieshima,K., Yoshie,O., Gabazza,E,C., Katayama,N., Shiku,H., Kuribayashi,K., and Kato,T. The Journal of Immunology 181:6889-6897,2008

2×GeneAce SYBR^® qPCR Mix α No   ROX

12.5 µL

Primers (5 µM each)

2.0 µL

DNA提取液(发根基因组DNA)

2.0 µL

d.d.H2O

up to 25.0 µL

95°C

10 min.

酶活性化处理

95°C

30 sec.

45次循环

60°C

1 min.

–

Isothermal Master Mix

15.0 µL

LAMP Primers

2.5 µL

DNA提取液(发根基因组DNA)

4.0 µL

d.d.H2O

up to 25.0 µL

扩增

63°C、60分钟

会合曲线分析

98°C-75°C、0.05°C/秒

ISOHAIR EASY

1,000 μL × 5瓶

-20°C