Phos-tag™ 质谱分析试剂盒

Phos-tag™ 质谱分析试剂盒
Phos-tag™ Mass Analytical Kit

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

Phos-tag™ Mass Analytical KitPhos-tag™ 质谱分析试剂盒

用于MALDI-TOF/MS检测,提高检测灵敏度!

  用于质谱分析的试剂套装。

  Phos-tag Mass Analytical Kit 是用于质谱分析的试剂套装,可配套MALDI-TOF/MS使用。可检测磷酸化分子- Phos-tag® 复合物,通常可提高低磷酸化分子的检测灵敏度。


试剂盒内容:

● Phos-tag™ MS-101L  5 mg([C27H29N6O64Zn2]3+ MW:581.4)

● Phos-tag™ MS-101H 5 mg([C27H29N6O68Zn2]3+ MW:589.4)

● Phos-tag™ MS-101N 10 mg([C27H29N6OZn2]3+ MW:584.3)

原理:


Phos-tag™ 质谱分析试剂盒


优点、特色:

● CH3COO- 等价结合在Phos-tag™ MS-101上。

● 在溶液中,不含有阴离子的Phos-tag™ MS-101 带有+3价。

● 检测前需制备1mM 的Phos-tag™ MS-101L,MS-101H或者MS-101N( 溶于水)。

案例、应用:

【使用例子:检测Phos-tag™ – 磷酸化LPA 复合体】


Phos-tag™ 质谱分析试剂盒


由于正电荷增大磷酸化LPA 检测灵敏度上升



Phos-tag™ 系列

磷酸化蛋白新方法!

  Phos-tag™ 是一种能与磷酸离子特异性结合的功能性分子。它可用于磷酸化蛋白的分离(Phos-tag™ Acrylamide)、Western Blot检测(Phos-tag™ Biotin)、蛋白纯化 (Phos-tag™ Agarose)及质谱分析MALDI-TOF/MS (Phos-tag™ Mass Analytical Kit)。


Phos-tag™ 的基本结构:

Phos-tag™ 质谱分析试剂盒

特点:

与-2价磷酸根离子的亲和性和选择性高于其它阴离子

在pH 5-8的生理环境下生成稳定的复合物

原理:


Phos-tag™ 质谱分析试剂盒

相关应用:


Phos-tag™ 质谱分析试剂盒

相关产品:

 产品名称

 用  途

 Phos-tag™ Acrylamide

 分离: SDS – PAGE 分离不同磷酸化水平的蛋白

 SuperSep Phos-tag™

 分离: 预制胶中含有50μM Phos-tag™ Acrylamide

 Phos-tag™ Biotin

 检测: 代替 Western Blot 检测中的磷酸化抗体

 Phos-tag™ Agarose

 纯化: 通用柱层析,纯化磷酸化蛋白

 Phos-tag™ Mass

 Analytical Kit

 分析: 用于质谱 MALDI-TOF/MS 分析,提高磷酸化分子的检测灵敏度


phos-tag™由日本广岛大学研究生院医齿药学综合研究科医药分子功能科学研究室开发。

更多产品信息,请点击:http://phos-tag.jp

Phos-tag™ 质谱分析试剂盒

Phos-tag 第5版说明书

Phos-tag™ 质谱分析试剂盒

Phos-tag系列 ver 5

Q.     Phos-tag™ Mass 用于实验可以使用多少次?

A.     如果每次用量为5μL,至少可以使用1000 次。

Q.     如何选择使用Phos-tag™ MS-101L,Phos-tag™ MS-101H 和Phos-tag™ MS-101N ?

A.     Phos-tag™ 101N 含有自然存在的Zn,101L 与101H 分别含有Zn 的同位素64Zn 和68Zn。

     请参考以下建议:

     摸索条件时使用101N,其中含有多种同位素,结果比较详细;

     鉴定磷酸基团是否存在,使用101L 和101H,这些试剂分别包含64Zn 和68Zn。使用这些试剂检测同一个样品

     时会产生不同的荷质比。

Q.     如果想测定经过Phos-tag™ SDS-PAGE 分离得到的样品,是否必须要在凝胶消化之前去除Phos-tag™?

A.     没有必要。SDS-PAGE 结束之后根据一般的凝胶消化方法进行操作即可。

Q.     能否用于ESI 质谱?

A.     是的,可以使用。请参考下面的文献,这篇报道使用Phos-tag™ MS-101N 进行ESI-MS 分析。在实验过程

     中,使用了中性溶液,若为酸性溶液会导致Phos-tag ™ 分离。

        【参考文献】 Anal. Chem. (2008), 80, 2531-2538 (MS-101N ESI-MS)

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·  Epithelial Sel1L is required for the maintenance of intestinal homeostasis[J]. Molecular biology of the cell, 2016, 27(3): 483-490, Sun S, Lourie R, Cohen S B, et al.

·  Effect of Sodium Dodecyl Sulfate Concentration on Supramolecular Gel Electrophoresis[J]. ChemNanoMat, 2016,Tazawa S, Kobayashi K, Yamanaka M.

·  Intergenic VNTR Polymorphism Upstream of rocA Alters Toxin Production and Enhances Virulence in Streptococcus pyogenes[J]. Infection and immunity, 2016: IAI. 00258-16,Zhu L, Olsen R J, Horstmann N, et al.

·  Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis[J]. Journal of Biological Chemistry, 2016: jbc. M116. 722751,Chen X, Stauffer S, Chen Y, et al.

·  Editorial: International Plant Proteomics Organization (INPPO) World Congress 2014[J]. Frontiers in Plant Science, 2016, 7,Heazlewood J L, Jorrín-Novo J V, Agrawal G K, et al.

·  Phosphoinositide kinase signaling controls ER-PM cross-talk[J]. Molecular biology of the cell, 2016, 27(7): 1170-1180,Omnus D J, Manford A G, Bader J M, et al.

·  A multiple covalent crosslinked soft hydrogel for bioseparation[J]. Chemical Communications, 2016, 52(15): 3247-3250,Liu Z, Fan L, Xiao H, et al.

·  Advances in crop proteomics: PTMs of proteins under abiotic stress[J]. Proteomics, 2016, 16(5): 847-865,Wu X, Gong F, Cao D, et al.

·  Cyclin-Dependent Kinase Co-Ordinates Carbohydrate Metabolism and Cell Cycle in S. cerevisiae[J]. Molecular cell, 2016, 62(4): 546-557,Zhao G, Chen Y, Carey L, et al.

·  Carbon Monoxide Gas Is Not Inert, but Global, in Its Consequences for Bacterial Gene Expression, Iron Acquisition, and Antibiotic Resistance[J]. Antioxidants & redox signaling, 2016,Wareham L K, Begg R, Jesse H E, et al.

·  Two-layer regulation of PAQR3 on ATG14-linked class III PtdIns3K activation upon glucose starvation[J]. Autophagy, 2016: 1-2,Xu D, Wang Z, Chen Y.

·  Regulation of sphingolipid biosynthesis by the morphogenesis checkpoint kinase Swe1[J]. Journal of Biological Chemistry, 2016, 291(5): 2524-2534,Chauhan N, Han G, Somashekarappa N, et al.

·  PAX5 tyrosine phosphorylation by SYK co-operatively functions with its serine phosphorylation to cancel the PAX5-dependent repression of BLIMP1: A mechanism for antigen-triggered plasma cell differentiation[J]. Biochemical and biophysical research communications, 2016, 475(2): 176-181,Inagaki Y, Hayakawa F, Hirano D, et al.

·  A Combined Computational and Genetic Approach Uncovers Network Interactions of the Cyanobacterial Circadian Clock[J]. Journal of Bacteriology, 2016: JB. 00235-16,Boyd J S, Cheng R R, Paddock M L, et al.

·  HuR mediates motility of human bone marrow-derived mesenchymal stem cells triggered by sphingosine 1-phosphate in liver fibrosis[J]. Journal of Molecular Medicine, 2016: 1-14,Chang N, Ge J, Xiu L, et al.

·  Combined replacement effects of human modified β-hexosaminidase B and GM2 activator protein on GM2 gangliosidoses fibroblasts[J]. Biochemistry and Biophysics Reports, 2016,Kitakaze K, Tasaki C, Tajima Y, et al.

·  Roseotoxin B Improves Allergic Contact Dermatitis through a Unique Anti-inflammatory Mechanism Involving Excessive Activation of Autophagy in Activated T-Lymphocytes[J]. Journal of Investigative Dermatology, 2016,Wang X, Hu C, Wu X, et al.


References on Phos-tag™ Chemistry

  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of phosphorylated compounds using a novel phosphate capture moleculeRapid Communications of Mass Spectrometry17, 2075-2081 (2003), H. Takeda, A. Kawasaki, M. Takahashi, A. Yamada, and T. Koike 

  • Recognition of phosphate monoester dianion by an alkoxide-bridged dinuclear zinc (II) complexDalton Transactions, 1189-1193 (2004), E. Kinoshita, M. Takahashi, H. Takeda, M. Shiro, and T. Koike

  • Quantitative analysis of lysophosphatidic acid by time-of-flight mass spectrometry using a phosphate capture molecule, Journal of Lipid Research45, 2145-2150 (2004), T. Tanaka, H. Tsutsui, K. Hirano, T. Koike, A. Tokumura, and K. Satouchi

  •  Production of 1,2-Didocosahexaenoyl Phosphatidylcholine by Bonito Muscle Lysophosphatidylcholine/TransacylaseJournal of Biochemistry,136, 477-483 (2004), K. Hirano, H. Matsui, T. Tanaka, F. Matsuura, K. Satouchi, and T. Koike

  • Novel immobilized zinc(II) affinity chromatography for phosphopeptides and phosphorylated proteins, Journal of Separation Science, 28, 155-162 (2005), E. Kinoshita, A. Yamada, H. Takeda, E. Kinoshita-Kikuta, and T. Koike

  • Detection and Quantification of On-Chip Phosphorylated Peptides by Surface Plasmon Resonance Imaging Techniques Using a Phosphate Capture MoleculeAnalytical Chemistry77, 3979-3985 (2005), K. Inamori, M. Kyo, Y. Nishiya, Y. Inoue, T. Sonoda, E. Kinoshita, T. Koike, and Y. Katayama

  • Phosphate-binding tag: A new tool to visualize phosphorylated proteins, Molecular & Cellular Proteomics, 5, 749-757 (2006), E. Kinoshita, E. Kinoshita-Kikuta, K. Takiyama, and T. Koike

  • Enrichment of phosphorylated proteins from cell lysate using phosphate-affinity chromatography at physiological pHProteomics, 6, 5088-5095 (2006), E. Kinoshita-Kikuta, E. Kinoshita, A. Yamada, M. Endo, and T. Koike

  • Separation of a phosphorylated histidine protein using phosphate affinity polyacrylamide gel electrophoresis, Analytical Biochemistry360, 160-162 (2007), S. Yamada, H. Nakamura, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, and Y. Shiro

  • Label-free kinase profiling using phosphate-affinity polyacrylamide gel electrophresisMolecular & Cellular Proteomics, 6, 356-366 (2007), E. Kinoshita-Kikuta, Y. Aoki, E. Kinoshita, and T. Koike

  • A SNP genotyping method using phosphate-affinity polyacrylamide gel electrophoresis, Analytical Biochemistry361, 294-298 (2007), E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike (The phosphate group at DNA-terminal is efficiently captured by Zn2+.Phos-tag.)

  • Identification on Membrane and Characterization of Phosphoproteins Using an Alkoxide-Bridged Dinuclear Metal Complex as a Phosphate-Binding Tag MoleculeJournal of Biomolecular Techniques18, 278-286 (2007), T. Nakanishi, E. Ando, M. Furuta, E. Kinoshita, E. Kikuta-Kinoshita, T. Koike, S. Tsunasawa, and O. Nishimura

  • A mobility shift detection method for DNA methylation analysis using phosphate affinity polyacrylamide gel electrophoresisAnalytical Biochemistry378, 102-104 (2008), E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike

  • Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate- affinity SDS-PAGEProteomics, 8, 2994-3003 (2008), E. Kinoshita, E. Kinoshita-Kikuta, M. Matsubara, S. Yamada, H. Nakamura, Y. Shiro, Y. Aoki, K. Okita, and T. Koike

  • FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathwayNature Structural & Molecular Biology15, 1138-1146 (2008), M. Ishiai, H. Kitao, A. Smogorzewska, J. Tomida, A. Kinomura, E. Uchida, A. Saberi, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, S. Tashiro, S. J. Elledge, and M. Takata

  • to Page top

  • Two-dimensional phosphate affinity gel electrophoresis for the analysis of phosphoprotein isotypes Electrophoresis30, 550-559 (2009), E. Kinoshita, E. Kinoshita-Kikuta, M. Matsubara, Y. Aoki, S. Ohie, Y. Mouri, and T. Koike

  • Formation of lysophosphatidic acid, a wound-healing lipid, during digestion of cabbage leavesBioscience, Biotechnology, and Biochemistry,73, 1293-300 (2009), T. Tanaka, G. Horiuchi, M. Matsuoka, K. Hirano, A. Tokumura, T. Koike, and K. Satouchi

  • A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phosphopeptidesAnalytical Biochemistry388, 235-241, (2009), K. Takiyama, E. Kinoshita, E. Kinoshita-Kikuta, Y. Fujioka, Y. Kubo, and T. Koike

  • Phos-tag beads as an immunoblotting enhancer for selective detection of phosphoproteins in cell lysatesAnalytical Biochemistry389, 83-85, (2009), E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike

  • Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agaroseProteomics9, 4098- 4101 (2009), E. Kinoshita, E. Kinoshita-Kikuta, H. Ujihara, and T. Koike

  • Separation and detection of large phosphoproteins using Phos-tag SDS-PAGENature Protocols4, 1513-1521 (2009), E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike

  • A clean-up technology for the simultaneous determination of lysophosphatidic acid and sphingosine-1-phosphate by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a phosphate-capture molecule, Phos-tagRapid Communications in Mass Spectrometry24, 1075-1084 (2010), J. Morishige, M. Urikura, H. Takagi, K. Hirano, T. Koike, T. Tanaka, and K. Satouchi

  • Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexesClinical Biochemistry43, 302-306 (2010), E. Kinoshita, E. Kinoshita-Kikuta, H. Nakashima, and T. Koike

  • The DNA-binding activity of mouse DNA methyltransferase 1 is ragulated phosphorylation with casein kinase 1σ/εBiochemical Journal427, 489-497 (2010), Y. Sugiyama, N. Hatano, N. Sueyoshi, I. Suetake, S. Tajima, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, and I. Kameshita


产品编号 产品名称 产品规格 产品等级 产品价格
305-93551 Phos-tag™ Mass Analytical Kit 
Phos-tag 质谱分析试剂盒
1 kit

PFA平底容器0104(容量:120ml)日本三博特sanplatec

PFA平底容器0104(容量:120ml)
产品编号: WEB15282 价格: 会员价:0元;市场价:0元 产品特点
使用温度范围:-196~200℃
产品规格

整体尺寸(mm)(外径×内径×高):49×  44×120

瓶盖尺寸(mm)(外径×高):59×124

材料

确认材料的耐药性 >> 耐药性检索

        PFA平底容器0104(容量:120ml)PFA平底容器0104(容量:120ml)产品特征

.特点:使用注射成型工艺,材质坚固,内底部平整,PFA 制,有优异耐药性,几乎不溶出金属离子,耐热性、耐寒性优异,可放入冰箱冷藏及放到油浴里加热, 使用温度范围广,(-196℃~200℃)。(底部平整,传热率优良。) ・同时具有不粘性,无须担心样品粘附。

作用:小瓶是无金属容量瓶,适合水质检查、ICP 分析等实验。 中瓶适合作为反应和保存药液的容器,大瓶适合作为反应用或保存强酸、有机溶剂等废弃液的容器。

注意:15280 ~ 15292 的瓶盖形状与照片不同。

 

30ml Oak Ridge离心管(外径×高(mm):25×92)日本三博特sanplatec

30ml Oak Ridge离心管(外径×高(mm):25×92)
产品编号: WEB18344 价格: 会员价:0元;市场价:0元 产品特点
通用规格,适合主流离心机使用。
产品规格

外径(mm):25

高(mm):92

个数:10

材料

确认材料的耐药性 >> 耐药性检索

        30ml Oak Ridge离心管(外径×高(mm):25×92)30ml Oak Ridge离心管(外径×高(mm):25×92)产品特征: .高速离心用的o型环请另外购买。
通用规格,适合主流离心机使用。
采用的树脂原料是Herolab公司的PF树脂(Polyflor)。耐热温度为-100℃~+140℃。 

生长调节剂等

生长调节剂等

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

生长调节剂

 

产品编号

产品名称

规格

容量

359-20561

1,2-O-Isopropylidene-α-D(-)-xylofuranose  ≧97.0%
  1,2-O-异亚丙基-alpha-D-呋喃木糖

Wako

5g

357-20562

25g

056-05661

Ergosterol
  ≧85.0%
  麦角固醇

化学用

1g

052-05663

5g

054-05662

25g

135-14411

Methyl Jasmonate  ≧85.0%
  茉莉酮酸甲酯

生化学用

5mL

133-14412

25mL

201-20171

1-Triacontanol

植物组织培养用

100mg

306-02871

Brassicasterol  ≧97.0%<br>
  菜籽甾醇

Tama Biochemical

100mg

322-56502

Phloroglucinol (Phloroglucinol,   Anhydrous)

Wako

25g

326-56505

500g

 


■CEP(C-Terminally Encoded Peptide)


促进植物氮素吸收的新肽键

  近年来,分泌型短肽在植物形态的形成和环境应答方面的重要作用已经逐渐显著1)。C-Terminally Encoded Peptide(CEP1)是分泌型短肽之一,在植物体中担任抑制氮素吸收的作用2)。

  植物从其根部将CEP1吸收到体内,使植物体内硝酸运转量成倍增长,从而促进硝酸因子的吸收3)。

[参考文献]:

 1) Annu.Rev.Plant Biol.,65,385(2014).

 2) Plant J.,55,152(2008).

 3) Science,346,343(2014).


产品编号

产品名称

规格

容量

332-44871

CEP1(C-Terminally Encoded Peptide)

0.1mg

 

 

■Phytosulfokine


含硫酸化酪氨酸的植物生长激素

  1996年,名古屋大学的松林、坂神先生从芦笋培养基中分离发现了世界上第一个肽类植物激素——Phytosulfokine1)。其分子内2个残留物翻译后会受硫酸化,这个硫酸化过程是生理活性的所必须的。

  Phytosulfokine对植物体的1)纳摩尔浓度下对植物细胞的增殖、分化活性;2)叶绿素合成促进活性;3)不定根、芽的形成;4)不定胚的形成;5)抗病害性等方面都起着相关作用2-5)。

[参考文献]:

 1) Y.Matsubayasi and Y.Sakagami,Proc.Natl.Acad.Sci.U.S.A.,93,7623(1996).

 2) Y.Matsubayasi,et al.,Chem.Record.,6.356(2006).

 3) A.Bahyrycz,et al.,j.Pept.Sci.,13,787(2007).

 4) D.Igarashi,et al.,Plant J.,71,194(2012).

 5) N.Sthrwohldt,et al.,PLoS,6,e21054(2011).


产品编号

产品名称

规格

容量

337-32091

Z-Ala-Ala-Asn-MCA

5mg

335-32271

Ac-Glu-Ser-Glu-Asn-MCA

5mg

 

生长调节剂等

植物研究用试剂

产品编号 产品名称 产品规格 产品等级 产品价格
359-20561 1,2-O-Isopropylidene-α-D(-)-xylofuranose  ≧97.0%
 1,2-O-异亚丙基-alpha-D-呋喃木糖
5g Wako
357-20562 1,2-O-Isopropylidene-α-D(-)-xylofuranose  ≧97.0%
 1,2-O-异亚丙基-alpha-D-呋喃木糖
25g Wako
056-05661 Ergosterol ≧85.0%
 麦角固醇
1g 化学用
052-05663 Ergosterol ≧85.0%
 麦角固醇
5g 化学用
054-05662 Ergosterol ≧85.0%
 麦角固醇
25g 化学用
135-14411 Methyl Jasmonate  ≧85.0%
 茉莉酮酸甲酯 
5mL 生化学用
133-14412 Methyl Jasmonate  ≧85.0%
 茉莉酮酸甲酯 
25mL 生化学用
201-20171 1-Triacontanol 100mg 植物组织培养用
306-02871 Brassicasterol  ≧97.0%
 菜籽甾醇
100mg Tama Biochemical
322-56502 Phloroglucinol (Phloroglucinol, Anhydrous) 25g Wako
326-56505 Phloroglucinol (Phloroglucinol, Anhydrous) 500g Wako
CEP1(C-Terminally Encoded Peptide)
332-44871 CEP1(C-Terminally Encoded Peptide) 0.1mg
Phytosulfokine
337-32091 Z-Ala-Ala-Asn-MCA 5mg
335-32271 Ac-Glu-Ser-Glu-Asn-MCA 5mg

即用型彩色蛋白预染Marker,稳定型

即用型彩色蛋白预染Marker,稳定型
Protein MultiColor,Stable DynaMarker®

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献


即用型彩色蛋白预染Marker,稳定型即用型彩色蛋白预染Marker,稳定型

Protein MultiColor,Stable DynaMarker®


在4° C 下可以保存一年,即用型彩色蛋白预染Marker,天然蛋白经预染标记。通过独创的蛋白染色技术,实现高稳定性,可以保存于冰箱内。

即用型彩色蛋白预染Marker,稳定型


凝胶组成:5~20%聚丙烯酰胺梯度凝胶,37°C孵育3个星期                                                                                                                                                                                                              

 染色BSA


                        传统产品        本产品

即用型彩色蛋白预染Marker,稳定型

               不同染色法下蛋白稳定性的比较

                                                                                                                                                       

为了对比传统产品(DynaMarker® Protein MultiColorⅢ)以及使用本产品制备的染色蛋白(BSA)在4° C 的稳定状态,先在37° C 的严峻条件下孵育3 个星期,然后再观察SDS-PAGE 条带的状态。

  本产品制备的染色BSA,比传统产品更能保持条带的位置及形状,具有高稳定性。

 

产品编号 产品名称 产品规格 产品等级 产品价格
DM660S Protein MultiColor,Stable DynaMarker®
即用型彩色蛋白预染Marker稳定型
300μl
DM660 Protein MultiColor,Stable DynaMarker®
即用型彩色蛋白预染Marker稳定型
600 μl
DM660L Protein MultiColor,Stable DynaMarker®
即用型彩色蛋白预染Marker稳定型
5 x 600 μl

凹形接头(30系列)(简称:30- 8FC- 4C)日本三博特sanplatec

凹形接头(30系列)(简称:30- 8FC- 4C)
产品编号: WEB12254 价格: 会员价:0元;市场价:0元 产品特点
简称:30- 8FC- 4C
产品规格

.

材料

确认材料的耐药性 >> 耐药性检索

        凹形接头(30系列)(简称:30- 8FC- 4C)凹形接头(30系列)(简称:30- 8FC- 4C)产品特征

A

C

D

E

F

W

T(mm)

适用管外径(inch)

Rc(PT)

产品编号

简称

37

18

12

6

17

19

8

5/16

1/4

12254

30- 8FC- 4C

单位:mm

主体:PTFE

螺帽:ECTFE

规格里的“Rc(PT)”指管螺纹的规格。

W是对角线的尺寸。

特点

毫米、英寸规格的管均可通用 

60ml PFA接续容器(接口型号:1/4inch 接口2个,顶端及侧面各一个)日本三博特sanplatec

60ml PFA接续容器(接口型号:1/4inch 接口2个,顶端及侧面各一个)
产品编号: WEB20773 价格: 会员价:0元;市场价:0元 产品特点
适合检测气体,使用防变质的氟树脂制造。
产品规格

容量(ml< ?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />

60

接口型号

14inch 接口2个,顶端及侧面各一个

管径×高(mm

36.8×173.9

材料

确认材料的耐药性 >> 耐药性检索

        60ml PFA接续容器(接口型号:1/4inch 接口2个,顶端及侧面各一个)60ml PFA接续容器(接口型号:1/4inch 接口2个,顶端及侧面各一个)产品特征: 容器形状:平面
18329,18330,20773,20774:60ml   23027:375ml
●特点
.60ml的PFA长体罐子带有可通到底的管口。
.垂直端口可连续的收集样品。
.Ultem Clamp。不接触流体。
.适合气体的测试氟树脂的不活性性质防止在容器
表面的气体变质.消失。 

防静电PFA-NE管(10m) 9.52×12.7日本三博特sanplatec

防静电PFA-NE管(10m) 9.52×12.7
产品编号: WEB18513 价格: 会员价:0元;市场价:0元 产品特点
具备条纹状导电部位的PFA管。
产品规格

规格(外径×内径×厚度):9.52Φ×12.7  (1/2″)Φ×1.59

材料

确认材料的耐药性 >> 耐药性检索

        防静电PFA-NE管(10m) 9.52×12.7防静电PFA-NE管(10m) 9.52×12.7产品特征

※仅售标准尺寸(10m)
特点
.管表面的条纹状导电部位配备PFA管。
.导电性PFA屏蔽效应,由于在可燃性气体中管表面会火花放电,最适合预防火灾事件的发生。
.通过绝缘环境下的放电, 防止管绝缘破坏。
.与金属线、 金属网格相比, 没有腐蚀的担忧。
详述
最高使用温度 : 200℃
导电部位体积固有抵抗率 : 5.3×102(Ω ・ m)

 

47mm 聚碳酸酯制内置过滤器1119日本三博特sanplatec

47mm 聚碳酸酯制内置过滤器1119
产品编号: WEB21326 价格: 会员价:0元;市场价:0元 产品特点
适用于气体、液体中微粒样品,坚固轻量
产品规格
构成材料 主体:聚碳酸酯
软管适配器:尼龙
屏幕:聚苯基砜(PPS)
O型圈:硅(Si)
排气盖:聚丙烯(PP)
有效面积 9.6cm2
尺寸 全长:5.8cm
直径:6.4cm
入口/出口接合处形状 0.25英寸-18NPT、软管适配器使用于内径6.4mm的管子。
最大耐热度 过滤器和软管的耐热温度都是121℃。
最大耐压力 340kPa
灭菌方法 此品未灭菌。必要时请于121~123℃,100kPa下灭菌15~20分钟。
材料

确认材料的耐药性 >> 耐药性检索

        47mm 聚碳酸酯制内置过滤器111947mm 聚碳酸酯制内置过滤器1119产品特征

重量很轻,可用于空气监测。

带Luer Lock的阀门,可轻易除去液体过滤中的气泡。

可用于in-line的液体和气体过滤,同样也可用于室内和屋外的空气监测和采样。

 

 

O形圈接头(30系列)(简称:30-3MR1/4UC)日本三博特sanplatec

O形圈接头(30系列)(简称:30-3MR1/4UC)
产品编号: WEB14703 价格: 会员价:0元;市场价:0元 产品特点
简称:30-3MR1/4UC
产品规格
.
材料

确认材料的耐药性 >> 耐药性检索

        O形圈接头(30系列)(简称:30-3MR1/4UC)O形圈接头(30系列)(简称:30-3MR1/4UC)产品特征

A

B

C

D

ØE

F

G

ØH

ØJ

K

W

T (mm)

适用管外径(inch)

M

产品编号

简称

21

6

9

5

2

8

7

8

7

2

10

3

1/8

1/4-28UNF

14703

30-3MR1/4UC

单位:mm

主体:PTFE

螺帽:ECTFE

O形圈:氟橡胶

W是对角线的尺寸。

 

特点

毫米、英寸规格的管均可通用。

 

道康宁制药级硅胶软管 美国DOW CORNING 货号:AV05488

名称:道康宁制药级硅胶软管

品牌:美国DOW CORNING

订货号:AV05488

道康宁制药级硅胶软管                                                        美国DOW CORNING                                                        货号:AV05488

咨询此产品

产品介绍

· 柔韧性高

· 低溶出物,降低药物污染的风险

· 不含过氧化物副产品、氯苯基或多氯联苯(PCB)类化合物

· 不含有机可塑剂、增塑剂或乳胶添加剂

· 根据性能要求提供三种不同硬度

· 采用 SILASTIC?生物医药级弹性体制成,该弹性体超过美国药典(USP?)第六类塑料测试的要求

· 按照FDA 21 CFR 210/211 关于制药产品的现行药品生产质量管理规范(cGMPs)生产

· 生产工厂在FDA注册(CFN 1816403

Grand PFA烧杯 (可附手柄)

Grand PFA烧杯 (可附手柄)
Grand PFA烧杯

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Grand PFA烧杯 (可附手柄)


耐化学性、耐热性优异,不含金属的氟树脂制烧杯。

溶液和试剂都不易沾着,附手柄,使用便利。可使用磁力搅拌器搅拌。

Grand PFA烧杯 (可附手柄)

   

◆原理

 

      三博特独创品牌Grand PFA烧杯使用高品质「NEW PFA」制成的烧杯。配备30ml~2000ml 规格的产品。并可根据用途选择有手柄或没手柄的产品。旧PFA和NEW PFA的溶出氟离子比较,PFA 氟树脂的耐化学性和耐热性优异,常被用于半导体工艺里的晶圆制造及管道系统。Grand PFA烧杯的原材料——NEW PFA为LSI高密度、高集成后生成的周边材料,对应高纯度需要的高品质PFA。

Grand PFA烧杯 (可附手柄)

Grand PFA烧杯 (可附手柄)



◆优点特色

 

耐化学性、耐热性、耐寒性优越,酸、碱、有机溶剂等大部分液体都可使用。可直接放冰箱内冷藏或油浴加热。
无金属溶出不必担心样品被微量金属污染,适合微量分析。
不粘附样品,即使样品粘度高也很容易倒出无需特殊操作。从一般的试料到粘度高的试料都可以使用。
透明度超群。使用三博特独自研制的模具,Grand PFA 烧杯透明度比旧产品更出众
一体成型的手柄,以前的手柄由PTFE制,不能与烧杯溶为一体,新产品整个烧杯一体成形无需担心手柄裂开。设计适合人手抓拿,即使烧杯装满液体也不会握不住。
平坦的底面,杯底平滑,可放于涡旋搅拌器上使用。
鲜明的刻度,刻度突起,即使手湿也不怕打滑,同时刻度比旧产品更大,方便观察。
批次控制,全部产品的底面都有材料标识、生产日期等刻印,方便使用。
耐候性,很少受到紫外线的影响,很少变黄,变质,可长期维持好的品质。


Grand PFA烧杯 (可附手柄)

◆案例应用

适合微量分析。
高精度分析、半导体业界里使用、同时提供经过酸洗可抑制金属溶出的Clean package。


Grand PFA烧杯 (可附手柄)

三博特容器类产品单页

Q:    「铁氟龙烧杯」与「PFA烧杯」是由不同材料制造的吗?
A:    一般所说的「铁氟龙」就是「氟树脂」,但实际上「铁氟龙」是杜邦公司注册的商标名称。严格来说,

          「氟树脂」是合成树脂其中的一个分类,而「铁氟龙」是生产商使用的产品名。
          三博特(sanplatec)采用大金工业株式会社的「PFA」原料,与「铁氟龙」同样都为氟树脂。
    
Q:    PFA是什么?是氟树脂的一种吗?
A:    氟树脂是分子结构中含有氟原子的一类热塑性树脂。具有优异的耐高低温性能、介电性能、化学稳定性、

          耐候性、不燃性、不粘性和低的摩擦系数等特性。PFA是其中一种氟树脂,拥有优异的耐热性、耐药性、

          电学特性(高频率特性)、不粘着、自润性。
    
Q:    我现在正在使用PTFE铁氟龙烧杯。
          GrandPFA烧杯透明度比PTFE铁氟龙烧杯好,耐药性也可以跟PTFE相比吗?
A:    PTFE和PFA都是氟树脂的一种。
          两种材料都拥有几乎相同的耐药性和耐热性。而PFA的透明度比PTFE更好,更方便顾客使用。


产品编号 产品名称 产品规格 产品等级 产品价格
11110 Grand PFA烧杯 (附手柄) 100mL
11111 Grand PFA烧杯 (附手柄) 200mL
11112 Grand PFA烧杯 (附手柄) 500mL
11113 Grand PFA烧杯 (附手柄) 1L
11114 Grand PFA烧杯 (附手柄) 2L
11115 Grand PFA烧杯  30mL
11116 Grand PFA烧杯 50mL
11117 Grand PFA烧杯  100mL
11118 Grand PFA烧杯  200mL
11119 Grand PFA烧杯 300mL
11120 Grand PFA烧杯 500mL
11121 Grand PFA烧杯 1L

PTFE A型套筒(简称:A-4)日本三博特sanplatec

PTFE A型套筒(简称:A-4)
产品编号: WEB9510 价格: 会员价:0元;市场价:0元 产品特点
简称:A-4
产品规格

产品编号

简称

连接部位

9510

A-4

29/32

A型厚度0.4L,附有特殊加工环。

材料

确认材料的耐药性 >> 耐药性检索

        PTFE A型套筒(简称:A-4)PTFE A型套筒(简称:A-4)产品特征: ※A型厚度0.4mm,附经特殊加工的圈。
●特点
PTFE管套可在-100℃~250℃条件下长期使用。
无需润滑,滑动面稳定,防止固定粘合。 

NBR1 ELISA kit

NBR1 ELISA kit

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细胞自噬研究 NBR1 ELISA kit


       自噬(autophagy)是指从粗面内质网的无核糖体附着区脱落的双层膜包裹部分胞质和细胞内需降解的细胞器、蛋白质等成分形成自噬体(autophagosome),并与溶酶体融合形成自噬溶酶体,降解其所包裹的内容物,以实现细胞本身的代谢需要和某些细胞器的更新。自噬是继细胞凋亡(apoptosis)后,当前生命科学最热的研究领域。


自噬过程示意图

NBR1 ELISA kit

ELISA法定量检测自噬标记物——NBR1和p62
       NBR1和p62这两个蛋白的功能类似于支架蛋白,在自噬过程中起着转运和降解蛋白的功能。NBR1和p62 ELISA试剂盒可以灵敏地定量检测人、大鼠、小鼠细胞裂解液中自噬标记物NBR1和p62的含量。此试剂盒具有如下优点:

● 灵敏度高,检测NBR1可低至66pg/ml、p62可低至100pg/ml。

● 简便地操作步骤节省宝贵时间,减少错误。

● 结果比Western Blot更可靠。

● 适用于高通量筛选。

 

使用p62 ELISA试剂盒检测自噬标记物

图A图B图C

       自噬诱导药物处理人乳腺癌细胞。经药物处理6小时和12小时后,细胞裂解,使用p62 ELISA试剂盒定量检测、p62抗体和LC3-II抗体进行免疫印迹。经药物处理后,自噬标记物表达水平有变化,p62蛋白水平降低(A和B图),LC3-II水平上升(C图)

产品信息

 

产品编号

产品名称

规格

ADI-900-211

NBR1 ELISA Kit

1X96孔

ADI-900-212

p62 ELISA Kit

1X96孔

 

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ADI-900-106

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p53/MDM2

 

自噬化合物库
       Screen-well™ Autophagy Library是研究促自噬和抗自噬分子的有力工具。化合物库中包含96种已知的自噬诱导剂或者抑制剂,可作用于与自噬相关的蛋白或受体,包括:mTOR/PI3K、cAMP、热休克蛋白、钙调蛋白、表观遗传调控蛋白、细胞骨架等。

 

产品编号

产品名称

规格

BML-2837-0500

Autophagy Library

1 Library

500μl/well

BML-2837-0100

1 Library

100μl/well

 

相关化合物

    产品编号

产品名称

产品编号

产品名称

BML-CM110

Bafilomycin A1

BML-A275

Rapamycin

ALX-550-087

Clonidine

BML-EI397

SMER28

ALX-550-253

Loperamide

ALX-550-095

Tamoxifen

BML-PI102

MG-132

ALX-550-306

Verapamil

 

Cyto-ID™自噬检测试剂盒

 
       ENZO公司提供的新产品Autophagy Detection Kit内含ENZO申请专利的染料,在活细胞内可以染色,与自噬体特异性结合,在荧光显微镜下镜检。

NBR1 ELISA kit

NBR1 ELISA kit

Starvation诱导细胞自噬,左图绿色为自噬体,右图为对照。蓝色为荧光染料Hoechst染细胞核。

 

相关产品:

 

   产品编号

产品名称

规格

ENZ-51034

 Cyto-ID™ Green detection kit

1 Kit

ENZ-51005

 Cyto-ID™ Red detection kit (GFP-Certified™)

1 Kit

ENZ-51015

 Cyto-ID™ Red cytotoxicity kit (GFP-Certified™)

1 Kit

ENZ-52252

 MM 4-64 Fluorescent dye for autophagy in yeast

1mg

 

自噬蛋白抗体
 Enzo Life   Sciences提供大量抗体用于自噬研究,单抗、多抗;可识别多种物种:人、小鼠、大鼠等;应用于多种实验:IHC、WB、ICC、IP等。

产品编号

产品名称

规格

 ALX-803-080-C100

 LC3B,mAb(5F10)

100μg

 BML-PW1125-0025

 NBR1(human), pAb

25μl

 ALX-215-065-1

 mTOR(human FRB Domain), pAb

1 vial

 ADI-905-721-100

 Beclin-1, pAb

100μg

 ADI-905-687-100

 mTOR, pAb

100μg

 BML-PW9860-0025

 P62(human), pAb

25μl

产品编号 产品名称 产品规格 产品等级 产品价格
ADI-900-211 NBR1 ELISA kit 
NBR1酶联免疫试剂盒
1X96孔等

碱性磷酸酶染色试剂盒II

碱性磷酸酶染色试剂盒II

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碱性磷酸酶染色试剂盒II碱性磷酸酶染色试剂盒II



  胚胎干细胞(ES)和诱导多能干细胞(iPS)的未分化状态可以通过高水平的碱性磷酸酶(AP)表达来表征,所述碱性磷酸酶(AP)表达与其他表面标志物的表达一起表明具有自我潜力的未分化细胞-renew.AP是在碱性条件下负责脱磷酸化分子如核苷酸,蛋白质和生物碱的水解酶。 当使用AP染色试剂盒对固定的ES或iPS细胞染色时,未分化的细胞呈现红色或紫色,而分化的细胞呈现无色。


◆试剂盒组成


  固定溶液:  25 mL (Cat. No. 09-0042)

  AP染色溶液A:10mL(目录号09-0043) 
  AP染色溶液B:10mL(目录号09-0065) 

  AP染色溶液C:10mL(目录号09-0066)


储存和稳定性


  在4°C储存, 不要冻结。 


质量控制


  AP染色试剂盒II在人类和小鼠ES细胞上进行功能测试,以确保产品质量。

参考文献


[1] Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.
[2] Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351.
[3] Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
[4] Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.

产品编号 产品名称 产品规格 产品等级 产品价格
00-0055 Stemgent Alkaline Phosphatase Staining Kit II (50 assays) 
Stemgent碱性磷酸酶染色试剂盒II
50 assays

电导率测定用氯化钾

电导率测定用氯化钾

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电导率测定用氯化钾电导率测定用氧化剂

本产品是适用于JISK8121[电导率测定用]的氯化钾。现在追加25g包装。

实验项目

规格值

外观

白色、结晶~结晶型粉末

纯度(KCL)(500℃干燥后)

质量分数99.9%以上

水溶状态

适合实验

干燥减量(110℃

质量分数0.1%以下

pH(50g/L,25℃)

5.0~8.0

溴化物

适合实验

碘化物

适合实验

磷酸盐(PO4)

质量分数5ppm以下

硫酸盐(SO4)

质量分数0.002%以下

氮化合物(N)

质量分数0.001%以下

钠(Na)

质量分数0.02%以下

铜(Cu)

质量分数2ppm以下

镁(Mg)

质量分数0.002%以下

钙(Ca)

质量分数0.002%以下

钡(Ba)

质量分数0.001%以下

铅(Pb)

质量分数2ppm以下

铁(Fe)

质量分数2ppm以下

产品编号 产品名称 产品规格 产品等级 产品价格
162-17942 Potassium Chloride
 氯化钾
25g for Measurement of Electric Conductivity
166-17945 Potassium Chloride
 氯化钾
500g for Measurement of Electric Conductivity