BIOTOOL代理 Poly FlAG多肽The Poly DYKDDDDK （FLAG） Peptide lyophilized powder was synthesized by 23 amino acid residues with molecular weight 2,864 Da. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three
详细介绍
BIOTOOL代理 Poly FlAG多肽
Properties
Appearance (Color)
White
Appearance (Form)
Powder
Form
Lyophilized Powder
Purity by HPLC-MS
95.33 % (the latest lot) Poly FLAG Peptide lyophilized powder
Concentration
(Recommended working concentration is 100-400 μg/mL for elute FLAG fusion proteins from the Biotool Anti-DYKDDDDK (FLAG) affinity gel.
Shipped in
Blue ice
Storage Temperature
2-8°C
Application
For use in competitive elution of DYKDDDDK (FLAG) fusion proteins from the ANTI-FLAGmonoclonal antibody in solution or bound to agarose on the Biotool Anti-DYKDDDDK (FLAG) affinity gel.
Attention
1. Read the User Manual carefully before the first use; 2. Avoid freezing, drying and high-speed centrifugation during use and storage of beads; 3. This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Procedure
Peptide Elution of DYKDDDDK(FLAG) Fusion Protein from Biotool Anti-DYKDDDDK(FLAG) affinity gel
1. Thoroughly suspend the Anti-Flag Affinity Gel in the vial, for a uniform suspension of the resin. Quickly transfer 10μl of the gel suspension (about 5μl of packed gel volume) to a fresh tube.
2. Add 0.6 mL TBS. Thoroughly suspend the Anti-Flag Affinity Gel by pipetting. Centrifuge the resin at 5000 rpm for 30 secondsand carefully remove the supernatant. Be sure to remove all of the wash buffer without discarding the resin. Repeat 3-4 times.
3. Add 500 μL of cell periplasmic extracts to the washed resin.
4. Gently agitate samples for 2 hours at 4°C. 5. Centrifuge the resin for 30 seconds at 5000 rpm. Transfer the supernatants to a fresh tube. 6. Wash the resin with 0.5mL TBS until the OD280 of the supernatant reads＜0.05. 7. Elution of DYKDDDDK (FLAG) Fusion Protein by Competition with Poly DYKDDDDK (FLAG) Peptide. Elute the bound DYKDDDDK(FLAG) Fusion Proteinby competitive elution with five one-column volume aliquots of a solution containing 100-400 ug/mL Poly DYKDDDDK(FLAG) Peptide in TBS. 8. Recycling the Biotool Anti-DYKDDDDK(FLAG) affinity gel Poly DYKDDDDK(FLAG) Peptidemay not elute all of the DYKDDDDK(FLAG) Fusion Protein bound to Biotool Anti-DYKDDDDK(FLAG) affinity gel. It is recommended the Biotool Anti-DYKDDDDK(FLAG) Affinity gel be regenerated immediay after use by washing with three 5 mL aliquots of 0.1 M glycine HCl, pH 3.5. The gel should be immediay re-equilibrated in TBS until the effluent is at neutral pH. Note: Do not leave the Biotool Anti-DYKDDDDK(FLAG) Affinity gel in glycine HCl for longer than 20 minutes. 9. Storing the Biotool Anti-DYKDDDDK(FLAG) Affinity gel Wash the Biotool Anti-DYKDDDDK(FLAG) Affinity gel three times with 5 mL of 50% glycerol with 10mM sodium phosphate, 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide. then add another 5 mL of 50% glycerol with 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azideand store at -20°C without draining.
本酶催化在各种多聚核糖核酸的3′末端聚合A碱基的反应。反应中使酶达到高活性所需的盐浓度为300-400 mM NaCl。能以多种单链RNA作引物，双链RNA以及合成的多聚核苷酸、短的寡聚核苷酸等不宜作引物；DNA也不能作引物。本酶因聚合AMP碱基，故只能用ATP作底物，ADP、dATP均不能作为底物。另外，UTP、CTP的掺入不足ATP的5%，而GTP也不能作为底物进行聚合。
■ 保存
-20℃。
■ ATP的掺入个数和时间的关系
以1 mM ATP作底物，15 U的Poly(A) Polymerase作用于84 μg RNA所得到的结果如下：
Synonyms: Poly(sodium acrylate) or Sodium polyacrylate (C3H3NaO2)n Mw Average ~ 5,100 by GPC CAS Number [9003-04-7] RTECS WD6826000 MDL Number MFCD00147949 PubChem Substance ID 24868314
Measured Conductivity Range: 30.5 – 41.1 mS/cm at 25°C Measured Refractive Index Range: 1.40682 – 1.41073 at 20°C
同义词：聚（丙烯酸钠）或聚丙烯酸钠 (C3H3NaO2)n Mw 平均 ~ 5,100 GPC CAS 编号 [9003-04-7] RTECS WD6826000 MDL 编号 MFCD00147949 PubChem 物质 ID 24868314
1. Polymeric precipitants for the crystallization of macromolecules. Sam Patel, Bob Cudney and Alexander McPherson. Biochemical and Biophysical Research Communications, Vol. 207, No. 2, 1995.
2. Crystallization and preliminary X-ray analysis of ginkbilobin-2 from Ginkgo biloba seeds: a novel antifungal protein with homology to the extracellular domain of plant cysteine-rich receptor-like kinases. Takuya Miyakawa, Yoriko Sawano, Ken-ichi Miyazono, Ken-ichi Hatanob and Masaru Tanokura. Acta Cryst. (2007). F63, 737–739.
HR2-773 Poly(acrylic acid sodium salt) 5,100 SDS