GE电流等电点标准pH3-10Amersham Broad pI Kit17-0471-01

GE电流等电点标准pH3-10Amersham Broad pI Kit

  • 产品型号:  17-0471-01
  • 简单描述
  • GE电流等电点标准pH3-10Amersham Broad pI Kit现货*
详细介绍

GE电流等电点标准pH3-10Amersham Broad pI Kit

简答介绍:

Broad pI Kit

 

pl markers are lyophilized mixtures of stable, salt-free, highly purified proteins. The markers may be run in parallel with the experimental samples on isoelectric focusing gels. These markers are not prestained.

 

The precisely defined pI of the markers allows a permanent and accurate calibration of sample components. pH gradients and pI can be measured to within 0.01 to 0.05 pH unit. The progress of the focusing experiment can be monitored visually by the focusing of methyl red or cytochrome c.

GE电流等电点标准pH3-10Amersham Broad pI Kit

Complete Packsize 10 vials, 325 μg/vial
Order Information Not for use in denaturing (urea) systems.; Store at 4°C.
Application To be used for isoelectric focusing (IEF)
pI 3.5-9.31)
Components Amylglucosidase – 3.50 pI (Native)
Methyl red (dye) – 3.75 (pI Native)
Trypsin inhibitor – 4.55 (pI Native)
b-Lactoglobulin A – 5.20 (pI Native)
Carbonic anhydrase B (bovine) – 5.85 (pI Native)
Carbonic anhydrase B (human) – 6.55 (pI Native)
Myoglobin, acidic band – 6.85 (pI Native)
Myoglobin, basic band – 7.35 (pI Native)
Lentil lectin, acidic – 8.15 (pI Native)
Lentil lectin, middle – 8.45 (pI Native)
Lentil lectin, basic – 8.65 (pI Native)
Trypsinogen – 9.30 (pI Native)
Physical State Solid
Storage Conditions 4°C
Color White
Wetted Part No
1)Measured at 24°C


详细产品信息可和选购

Protein Molecular Weight Marker (Broad)酶试剂盒Takara


Protein Molecular Weight Marker (Broad)
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 3452Q Protein Molecular Weight Marker (Broad) 50 lanes ¥160 Protein Molecular Weight Marker (Broad) Protein Molecular Weight Marker (Broad) Protein Molecular Weight Marker (Broad)
Takara 3452 Protein Molecular Weight Marker (Broad) 200 lanes ¥360 Protein Molecular Weight Marker (Broad) Protein Molecular Weight Marker (Broad) Protein Molecular Weight Marker (Broad)
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■ 制品内容 (Code No.: 3452 约200 次量)Protein Molecular Weight Marker (Broad)
Protein MW Marker (Broad) 50 μl
5X Loading Buffer 1 ml
1 M DTT (Dithiothreitol) 100 μl
 
■ 浓度
18 μg/μl
 
■ 制品说明
Protein Molecular Weight Marker (Broad Range)是由九种纯化好的不同分子量的蛋白质组成的,它的分子量范围为:6.5 KDa~200 KDa。进行聚丙烯酰胺凝胶电泳后,经考马斯亮蓝R-250染色后的各种蛋白质的条带强度均一。本制品稀释20倍后进行聚丙烯酰胺凝胶电泳,以每个泳道取5 μl(for SDS-PAGE mini gel)电泳计算时,本制品约可使用200次。
 
■ 保存
Protein Molecular Weight Marker和1 M DTT可在-20℃下保存。
5X Loading Buffer使用后于室温保存。
 
■ 5-20%梯度SDS-PAGE电泳结果:
 
Protein Molecular Weight Marker (Broad)
 
■ 注意事项
推荐使用5-20%的聚丙烯酰胺梯度凝胶。浓度太低时,低分子量的蛋白迁移速度快于溴酚兰;浓度太高时,高分子量的蛋白分离效果不好,有可能聚集于分离胶的上部。
 
 

页面更新:2019-04-16 14:30:58