fermentasRNA酶抑制剂RiboLock RNase Inhibitor
- 产品型号： EO0381
- 简单描述
- fermentasRNA酶抑制剂RiboLock RNase Inhibitor应用范围广，适合多种使用条件。保护RNA，使其免受降解。Z高有效温度达55°C规格单位：2500U 4*2500U
详细介绍
fermentasRNA酶抑制剂RiboLock RNase Inhibitor
Description
Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.
Highlights
- Performs under a wide range of reaction conditions
- Protects RNA from degradation at temperatures up to 55°C (see Figure 2 in Supporting Data)
Note
DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity.
Specifications
| CategoryName |
|
| Concentration |
40 U/µL |
| Definition of Activity Unit |
- One unit of the RiboLock RNase Inhibitor inhibits the activity of 5 ng RNase A by 50%.
- Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/mL BSA, 100 ng/mL RNase A, 0.1 mg/mL [3H]-RNA, 50 mg/mL yeast RNA, 8 mM DTT.
|
| Hazardous |
No |
| Hazardous: |
No |
| Inactivation |
Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min heating at 70°C. |
| Inhibition |
Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound. |
| Molecular Weight |
49.6 kDa monomer |
| Quality Control |
- The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases confirmed by appropriate quality tests.
- Functionally tested in RNA and cDNA synthesis.
|
| Shelf Life: |
|
| Shipping Condition: |
|
| Shipping Information |
|
| Source |
E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor. |
| SpecificationName |
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| SpecificationValue |
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| Storage Buffer |
The enzyme is supplied in 20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.03% v/v) ELUGENT Detergent and 50% (v/v) glycerol. |
| Storage Condition |
-20 C |
|
Storage Condition:
|
fermentasRNA酶抑制剂RiboLock RNase Inhibitor *
*明细：
|
DreamTaq Green PCR Master Mix (2×)
|
K1081
|
200rxns
|
500
|
300
|
|
K1082
|
1000rxns
|
2,000
|
1200
|
|
DreamTaq PCR Master Mix (2×)
|
K1071
|
200rxns
|
500
|
300
|
|
K1072
|
1000rxns
|
2,000
|
1200
|
|
PCR master Mix (2×)
|
K0171
|
200rxns
|
450
|
276
|
|
K0172
|
1000rxns
|
1,800
|
1080
|
|
Long PCR Enzyme Mix
|
K0181
|
100U
|
250
|
150
|
|
K0182
|
500U
|
1,000
|
600
|
|
DreamTaq DNA Polymerase
|
EP0701
|
200U
|
150
|
90
|
|
EP0702
|
500U
|
200
|
120
|
|
EP0703
|
5x500U
|
900
|
540
|
|
EP0704
|
20x500U
|
3,200
|
1920
|
|
DreamTaq Green DNA Polymerase
|
EP0711
|
200U
|
150
|
90
|
|
EP0712
|
500U
|
200
|
120
|
|
EP0713
|
5x500U
|
1,080
|
648
|
|
EP0714
|
20x500U
|
3,200
|
1920
|
|
TurboFect in vitro Transfection Reagent
|
R0531
|
1ml
|
2,000
|
1300
|
|
TurboFect siRNA Transfection Reagent
|
R1401
|
0.5ml
|
1,600
|
1040
|
|
TurboFect Protein Transfection Reagent
|
R1411
|
125ul
|
2,140
|
1391
|
|
TurboFect in vivo Transfection Reagent
|
R0541
|
120ul
|
3,000
|
1950
|
|
| |
|
“赠送送到Hi”
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品名
|
货号
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包装
|
目录价￥
|
赠送说明
|
|
RevertAid First Strand cDNA Synthesis Kit
|
K1622
|
100rxns
|
1,600
|
买2个，赠1个K1081（DreamTaq Green PCR Master Mix），价值￥500
|
|
Maxima First Strand cDNA Synthesis Kit
|
K1641
|
50rxns
|
2,500
|
同时购买，赠2个K1081（DreamTaq Green PCR Master Mix），价值￥1000
|
|
GeneJET RNA Purification Kit
|
K0731
|
50preps
|
1,900
|
|
Maxima First Strand cDNA Synthesis Kit
|
K1642
|
200rxns
|
7,500
|
同时购买，赠2个K1082（DreamTaq Green PCR Master Mix），价值￥4000
|
|
GeneJET RNA Purification Kit
|
K0732
|
250preps
|
7,600
|
|
RevertAid Premium First Strand cDNA Synthesis Kit
|
K1651
|
50rxns
|
970
|
同时购买，赠2个K1081（DreamTaq Green PCR Master Mix），价值￥1000
|
|
GeneJET RNA Purification Kit
|
K0731
|
50preps
|
1,900
|
|
RevertAid Premium First Strand cDNA Synthesis Kit
|
K1652
|
200rxns
|
3,880
|
同时购买，赠2个K1082（DreamTaq Green PCR Master Mix），价值￥4000
|
|
GeneJET RNA Purification Kit
|
K0732
|
250preps
|
7,600
|
|
Maxima Reverse Transcriptase
|
EP0741
|
2000U
|
450
|
买1个，赠1个EO0381（RiboLock RNase Inhibitor），价值￥200
|
|
EP0742
|
10000U
|
1450
|
买1个，赠2个EO0381（RiboLock RNase Inhibitor），价值￥400
|
|
RevertAid Premium Reverse Transcriptase
|
EP0731
|
2000U
|
420
|
买1个，赠1个EO0381（RiboLock RNase Inhibitor），价值￥200
|
|
EP0732
|
10000U
|
1400
|
买1个，赠2个EO0381（RiboLock RNase Inhibitor），价值￥400
|
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