高灵敏性的检测表达基因。

使用KAPA Stranded mRNA-Seq制备的文库，与 I 系列产品制备同类型文库相比有着更低的重复率，保持了链特异性的同时，更多特异的转录本和特异的基因被检出。

覆盖均一性提升。

500 ng高质量RNA使用KAPA Stranded mRNA-Seq Kit和 I 系列产品进行文库制备，KAPA文库制备试剂盒在5’和3 端’位置测序的均一性更好。

富含GC转录本的覆盖度得到提升。

DVL3转录本的5’和3’端位置外显子区域(红色)为高GC含量区域。使用KAPA StrandedmRNA-Seq试剂盒(绿色)测序结果与 I 系列产品(橙色)结果相比，绿色区域有着更高的测序覆盖度。

低表达的转录本的覆盖度提升。

使用KAPA Stranded mRNA-Seq试剂盒(绿色)与 I 系列产品(橙色)进行文库制备，KAPA在GLTPD1基因上的覆盖度更高，即使测序深度相同， I 系列产品覆盖度仍有缺失(红色区域)。

产品手册

Shipping and storage

The enzymes provided in this kit are temperature sensitive, and appropriate care should be taken during shipping and storage. KAPA Stranded RNA-Seq Library Preparation Kits are shipped on dry ice or ice packs, depending on the destination country. Upon receipt, immediately store enzymes and reaction buffers at -20˚C in a constanttemperature freezer. The PEG/NaCl SPRI^® Solution should be protected from light, and stored at -20˚C. For shortterm use, the PEG/NaCl SPRI^® Solution may be stored protected from light at 4˚C for up to 2 months. When stored under these conditions and handled correctly, the kit components will retain full activity until the expiry date indicated on the kit label.

Handling

Always ensure that components have been fully thawed and thoroughly mixed before use. Keep all reaction components and master mixes on ice whenever possible during handling. KAPA HiFi HotStart ReadyMix (2X) may not freeze completely, even when stored at -20 °C. Nevertheless, always ensure that the KAPA HiFi HotStart ReadyMix is fully thawed and thoroughly mixed before use. PEG/NaCl SPRI^® Solution does not freeze at -20 °C, but should be equilibrated to room temperature and mixed thoroughly before use.

Quality control

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exoand endonuclease activities, and meet strict requirements with respect to DNA contamination. Please contact support@kapabiosystems.com for more information.