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N-Glycan Sequencing Kit(已停产） 收藏
- isoschizomers |
- compatible ends |
- single letter code
N-glycan sequencing of antibodies
The enzymes provided are all active in a universal buffer system, GlycoBuffer 1, and can be used in
single digests or in any combination.
All reagents supplied are mass spectrometry compatible.
The N-Glycan Sequencing Kit consists of seven well characterized, highly pure, recombinant
exoglycosidase enzymes selected to simplify the process of characterizing typical N-linked glycan
structures. The extreme diversity of glycan structures on a glycoprotein makes elucidation of the profile
challenging and often a number of orthogonal approaches are employed to verify the individual structures.
The use of sequential or tandem exoglycosidase digestion of oligosaccharides followed by mass
spectrometry or capillary electrophoresis analysis provides detailed carbohydrate sequence information
and resolves ambiguities. The exoglycosidases are all active in a universal buffer system, GlycoBuffer 1,
and can be used in single digests or in combination.
This kit is compatible with N-linked glycans released from a variety of CHO and murine derived antibodies,
as well as N-linked glycans released from other glycoproteins. Optimal incubation times and enzyme
concentration may vary for more complex glycans. The recommended enzyme quantity is sufficient for
digestion of up to 130 pmol of released glycans (equivalent to released glycans from approximately 10 µgs
of antibody) labeled with 2AA, 2AB or procainamide. Other commercially available “Instant labels” may
require a larger quantity of enzyme or a longer incubation time for complete digestion.
α2-3,6,8,9 Neuraminidase A
α2-3 Neuraminidase S
β1-4 Galactosidase S
α1-2,4,6 Fucosidase O