Shibayagi 小鼠瘦素 ELISA试剂盒

Shibayagi 小鼠瘦素 ELISA试剂盒
Lbis® Leptin-Mouse

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Lbis® Leptin-MouseShibayagi 小鼠瘦素 ELISA试剂盒

Shibayagi 小鼠瘦素 ELISA试剂盒

  瘦素(Leptin)是一种分子量为16KD的蛋白质类激素。主要由白色脂肪组织产生,其它组织如褐色脂肪组织、胎盘、卵巢、骨骼肌、胃底部、乳腺上皮细胞、骨髓、下垂体、肝脏等也能检测到。其在大鼠和小鼠中有97%的同源性。瘦素在突变种肥胖小鼠中发现,是通过调节食欲和新陈代谢来参与至能量平衡的一种重要的激素。血液中瘦素的浓度会因为空腹和低热量饮食而降低,但进食后并不会有明显增加,只有肥胖即脂肪组织量增大的情况下才会增加,可反映身体脂肪量。瘦素进入大脑,通过抑制体内下丘脑神经肽Y(NPY)和刺鼠基因相关蛋白(AgRP)表达神经的受体,另外活化体内α-MSH表达神经,起到降低食欲,减少进食的作用。但是,对于严重肥胖的人,虽然他们血液中瘦素的浓度很高,但对瘦素有抗性,瘦素并不能发挥抑制食欲的作用。全身性脂肪萎缩病引起的瘦素降低或缺乏,会造成胰岛素抗性、糖尿病、脂肪肝、高甘油三酯血症等疾病。除此之外,瘦素也被认为与对动脉粥样硬化的免疫反应、能量平衡和性周期相关,与肺泡表面活性剂的产生也有关系。

◆特点

 • 短时间测定(总的反应时间:3小时)

 • 微量样品(标准操作:10 μL)可测

 • 使用对环境无害的防腐剂

 • 全部试剂均为液体,可直接使用

 • 精密的测定精度和高再现性

◆构成

 

组成

状态

容量

(A)抗体固相化 96 孔板(干燥孔板)

洗净后使用

96 wells(8×12)/1 块

(B)瘦素标准溶液(小鼠)(5,000 pg/ml)

稀释后使用

500 μL/1 瓶

(C)缓冲液

即用

60 mL/1 瓶

(D)生物素结合抗瘦素抗体

稀释后使用

200 μL/1 瓶

(E)过氧化物・抗生物素蛋白结合物

稀释后使用

200 μL/1 瓶

(F)显色液(TMB)

即用

12 mL/1 瓶

(H)反应终止液(1M H2SO4)※小心轻放

即用

12 mL/1 瓶

( I )浓缩洗净液(10×)

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

◆交叉反应

 ※交叉反应是浓度为3,000 pg/mL时的数据

动物种类

对象物质

反应性和反应率(%)

Mouse

Leptin

100

α-MSH

IFN-γ

MCH

TNF-α

Rat

Leptin

31.5

Human

Lepin

+:存在交叉反应

―:不存在交叉反应

◆样品信息

小鼠的血清、血浆

10 μL/well(标准操作)

※样品量在10~50 μL范围内可以配制

 

◆测量范围

20.6~5,000 pg/mL(样品量50μL

103~25,000 pg/mL(样品量10μL

◆Validation data

精度测试(检测内变动系数)

样品

A

B

C

1

3818

846

405

2

3810

856

405

3

3979

842

394

4

4047

851

392

5

4046

856

420

mean

3940

850

403

SD

118

6.18

11.2

CV(%)

3.0

0.73

2.8

单位:pg/mL

再现性测试(检测内变动系数)

测量日/样品

D

E

第0天

5007

1052

第1天

5126

1063

第2天

5069

1027

第3天

5000

1000

mean

5051

1035

SD

59.3

28.0

CV(%)

1.2

2.7

单位:pg/mL n=2

添加回收测试

样品F

添加量

实测值

回收量

回收率(%)

0.00

994.9

337

1334.0

339.1

101

1061

2099.0

1104

104

1238

2284.0

1289

104

单位:pg/mL n=2

样品G

添加量

实测值

回收量

回收率(%)

0.00

1996

2731

4741

2745

101

4682

6444

4448

95.0

5462

7717

5721

105

单位:pg/mL n=2

稀释直线性测试

用稀释缓冲液分5次连续稀释2个血清样品的测量结果,线性回归方程的R2在0.9992~0.9997之间。

欲了解更多相关产品信息,请点击文字:Lbis® 疾病相关动物模型ELISA试剂盒系列

相关资料


Shibayagi 小鼠瘦素 ELISA试剂盒 Shibayagi 小鼠瘦素 ELISA试剂盒 Shibayagi 小鼠瘦素 ELISA试剂盒
说明书

ELISA试剂盒选择指南①②

ELISA试剂盒选择指③④

参考文献



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产品编号 产品名称 产品规格 产品等级 产品价格
631-10389  (AKRLP-011)Lbis® Mouse Leptin ELISA kit
Lbis®小鼠瘦素 ELISA试剂盒 
96 tests