Shibayagi 大鼠胰岛素 ELISA试剂盒(T型)

Shibayagi 大鼠胰岛素 ELISA试剂盒(T型)
Lbis® Insulin-Rat-T

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  • 参考文献

Lbis® Insulin-Rat-TShibayagi 大鼠胰岛素 ELISA试剂盒(T型)

Shibayagi 大鼠胰岛素 ELISA试剂盒(T型)

  胰岛素是由胰脏内的胰岛β细胞分泌,分子量约5800,等电点在5.4左右的一种蛋白质激素。

  A6-A11、A7-B7、A20-B-19之间形成二硫键,在酸性溶液或者不含Zn离子的中性水溶液中形成二聚体,在含锌离子的中性溶液中,则形成含2个Zn离子的六聚体。

  肝脏、肌肉、脂肪组织是主要的靶组织,分别有以下的作用。

肝脏:促进糖原、蛋白质、脂肪酸合成、促进糖类的摄取和利用、抑制糖异生。

肌肉:糖类、氨基酸、K细胞膜通透性增大、促进糖原、蛋白质的合成、抑制蛋白质分解。

脂肪组织:葡萄糖细胞膜通透性增大、促进脂肪酸的合成。

  胰岛素是细胞内的合成单链胰岛素原通过二硫键结合一起形成的。在酶分解作用下被激活,C肽和胰岛素分离。

◆特点

● 短时间测定(总的反应时间:3小时)

● 微量样品(标准操作:10 μL)可测

● 使用对环境无害的防腐剂

● 全部试剂均为液体,可直接使用

● 精密的测定精度和高再现性

● 操作简便,不需要特别的预处理

● 有效期限为12个月

◆构成

组成

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96   wells(8×12)/1 块

(B) 胰岛素标准溶液(大鼠)(200 ng/mL)

稀释后使用

25 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

10 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

20 μL/1 瓶

(F) 显色液(TMB)

即用

12 mL/1 瓶

(H) 反应终止液(1M H2SO4)※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液(10×)

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

 

◆样品信息

大鼠的血清•血浆•培养液

10 μL/well(标准操作)

※血浆采血建议使用肝素处理血液

◆测量范围

0.156~10 ng/mL(标准曲线范围)

◆Validation data

精度测试(检测内变动系数)

 

样品

A

B

C

D

1

0.589

1.211

2.600

4.991

2

0.568

1.228

2.600

4.971

3

0.568

1.228

2.532

5.036

4

0.557

1.211

2.538

5.026

5

0.557

1.253

2.582

4.925

6

0.578

1.220

2.563

4.880

7

0.578

1.228

2.618

5.031

8

0.536

1.228

2.618

4.885

mean

0.566

1.226

2.581

4.968

SD

0.0165

0.0131

0.0340

0.0645

CV(%)

2.92

1.07

1.32

1.30

单位:ng/mL

 

 

再现性测试(检测内变动系数)

 

测量日/样品

E

F

G

第0天

6.74

3.31

1.16

第1天

6.69

3.25

1.22

第2天

6.23

3.21

1.21

mean

6.55

3.25

1.20

SD

0.2792

0.0479

0.0325

CV(%)

4.3

1.5

2.7

单位:ng/mL n=5

 

 

添加回收测试

 

样品H

添加量

理论值

实测值

回收率(%)

0

0.996

0.500

1.496

1.484

99.2

1.000

1.996

2.048

103

2.000

2.996

2.779

92.7

单位:ng/mL

 

样品I

添加量

理论值

实测值

回收率(%)

0

1.086

0.500

1.586

1.562

98.5

1.000

2.086

2.061

98.8

2.000

3.086

2.753

89.2

单位:ng/mL

 

样品J

添加量

理论值

实测值

回收率(%)

0

1.160

0.500

1.660

1.637

98.6

1.000

2.160

2.054

95.1

2.000

3.166

2.963

93.6

单位:ng/mL

 

 

稀释直线性测试

 

用稀释缓冲液分4次连续稀释2个血清样品的测量结果,直线回归方程的R2在0.9983~0.9992之间。

相关资料


Shibayagi 大鼠胰岛素 ELISA试剂盒(T型) Shibayagi 大鼠胰岛素 ELISA试剂盒(T型) Shibayagi 大鼠胰岛素 ELISA试剂盒(T型)
说明书

ELISA试剂盒选择指南①②

ELISA试剂盒选择指③④

参考文献


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61.

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62.

 Effects of Sleeve Gastrectomy on Lipid Metabolism in an Obese Diabetic Rat Model. Kawano, Y., Ohta, M., Hirashita, T., Masuda, T., Inomata, M., Kitano. S. Obesity Surgery, Vol.23(12), p1947-1956, Dec 2013.


63.

 Effects of Sleeve Gastrectomy on Lipid Metabolism in an Obese Diabetic Rat Model. Kawano, Y., Ohta, M., Hirashita, T., Masuda, T., Inomata, M., Kitano. S. Obesity Surgery, Vol.23(12), p1947-1956, Dec 2013.


64.

Effects of electrical microstimulation of peripheral sympathetic nervous fascicle on glucose uptake in rats. Sato D., Shinzawa G., Kusunoki M., Matsui T., Sasaki H., Feng Z., Nishina A., Nakamura T. Journal of Artificial Organs, Vol.16(3), p352-358, Sep 2013.


65.

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66.

Effects of Sleeve Gastrectomy on Lipid Metabolism in an Obese Diabetic Rat Model. Y.Kawano, M.Ohta, T.Hirashita, T.Masuda, M.Inomata, S.Kitano. Obesity Surgery, Jul 2013.


67.

Glucose Use in Fasted Rats Under Sevoflurane Anesthesia and Propofol Anesthesia. K.Sato, T.Kitamura, G.Kawamura, Y.Mori, R.Sato, Y.Araki, Y.Yamada. Anesth Analg, Jun 2013.


68.

Oral administration of corn Zein hydrolysate stimulates GLP-1 and GIP secretion and improves glucose tolerance in male normal rats and Goto-Kakizaki rats. N.Higuchi, T.Hira, N.Yamada and H.Hara. Endocrinology, Jun 2013.


69.

Pancreatic stellate cells reduce insulin expression and induce apoptosis in pancreatic β-cells. K.Kikuta, A.Masamune, S.Hamada, T.Takikawa, E.Nakano, T.Shimosegawa. Biochemical and Biophysical Research Communications, Vol.433(3), p292-297, Apr 2013.


70.

Pancreatic stellate cells reduce insulin expression and induce apoptosis in pancreatic β-cells. Kikuta K, Masamune A, Hamada S, Takikawa T, Nakano E, Shimosegawa T. Biochemical and Biophysical Research Communications,Available online 13, Mar 2013


71.

Hypothalamic Brain-Derived Neurotrophic Factor Regulates Glucagon Secretion Mediated by Pancreatic Efferent Nerves. Gotoh K, Masaki T, Chiba S, Ando H, Fujiwara K, Shimasaki T, Mitsutomi K, Katsuragi I, Kakuma T, Sakata T, Yoshimatsu H. Journal of Neuroendocrinology, Vol.25(3), p302-311, Mar 2013.


72.

Effects of electrical microstimulation of peripheral sympathetic nervous fascicle on glucose uptake in rats. Sato D, Shinzawa G, Kusunoki M, Matsui T, Sasaki H, Feng Z, Nishina A, Nakamura T. Journal of Artificial Organs, Mar 2013.


73.

Improvement of erectile function by Korean red ginseng (Panax ginseng) in a male rat model of metabolic syndrome. Kim S-D, Kim Y-J, Huh J-S, Kim S-W and Sohn D-W. Asian Journal of Andrology , Feb 2013.


74.

Quercetin intake during lactation modulates the AMP-activated protein kinase pathway in the livers of adult male rat offspring programmed by maternal protein restriction. Sato S., Mukai Y., Saito T. The Journal of Nutritional Biochemistry, Vol.24(1), p118-123, Jan 2013.


75.

Reduction of reactive oxygen species ameliorates metabolism-secretion coupling in islets of diabetic GK rats by suppressing lactate overproduction. Sasaki M, Fujimoto S, Sato Y, Nishi Y, Mukai E, Yamano G, Sato H, Tahara Y, Ogura K, Nagashima K and Inagaki N. Diabetes, January 24, 2013 , In press.


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Derangement of ghrelin secretion after long-term high-fat diet feeding in rats. Sugiishi A, Kimura M, Kamiya R, Ueki S, Yoneya M, Saito Y, Saito H. Hepatology Research, 2013, In press.

 

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Proteomic and bioinformatic analysis of membrane proteome in type 2 diabetic mouse liver. Kim G-H, Park E C, Yun S-H, Hong Y, Lee D-G, Shin E-Y, Jung J, Kim Y H, Lee K-B, Jang I-S, Lee Z-W, Chung Y-H, Choi J-S, Cheong C, Kim S, Kim S II. PROTEOMICS, 2013, In press.


78.

A Novel Rat Model of Type 2 Diabetes: The Zucker Fatty Diabetes Mellitus ZFDM Rat. Yokoi N, Hoshino M, Hidaka S, Yoshida E, Beppu M, Hoshikawa R, Sudo K, Kawada A, Takagi S and Seino S. Journal of Diabetes Research, Vol.2013 (2013)


79.

Urinary cystatin C as a biomarker for diabetic nephropathy and its immunohistochemical localization in kidney in Zucker diabetic fatty (ZDF) rats. Togashi Y, Miyamoto Y. Experimental and Toxicologic Pathology,Available online 12 Jul 2012.


80.

Artemisia campestris leaf extract alleviates early diabetic nephropathy in rats by inhibiting protein oxidation and nitric oxide end products. Mediha S, Hamadi F, Nejla S, Yassine C, Mohamed M, Najiba Z. Pathology – Research and Practice, Vol.208(3), p157-162, Mar 2012.


81.

Fenugreek with reduced bitterness prevents diet-induced metabolic disorders in rats. Muraki E, Chiba H, Taketani K, Hoshino S, Tsuge N, Tsunoda N and Kasono K. Lipids in Health and Disease, Vol.11(58), 2012.


82.

Oral Ingestion of Aloe vera Phytosterols Alters Hepatic Gene Expression Profiles and Ameliorates Obesity-Associated Metabolic Disorders in Zucker Diabetic Fatty Rats. E. Misawa., M. Tanaka., K. Nomaguchi., K. Nabeshima., M. Yamada., T. Toida., and K. Iwatsuki. J. Agric. Food Chem., 2012, 60 (11), pp 2799-2806


83.

 Myocardial Infarction-Prone Watanabe Heritable Hyperlipidemic Rabbits with Mesenteric Fat Accumulation Are a Novel Animal Model for Metabolic Syndrome. M. Shiomi., T. Kobayashi., N. Kuniyoshi., S. Yamada., T. Ito. Pathobiology 2012;Vol. 79 No. 6 P329-338


84.

High-fat diet-induced reduction of peroxisome proliferator-activated receptor-γ coactivator-1α messenger RNA levels and oxidative capacity in the soleus muscle of rats with metabolic syndrome. F. Nagatomo., H. Fujino., H. Kondo., I. Takeda., K. Tsuda., A. Ishihara. Nutrition Research, Vol. 32, Issue 2, February 2012, Pages 144-151


85.

The effects of running exercise on oxidative capacity and PGC-1α mRNA levels in the soleus muscle of rats with metabolic syndrome. F. Nagatomo., H. Fujino., H. Kondo., M. Kouzaki., N. Gu., I. Takeda., K. Tsuda., and A. Ishihara. The Journal of Physiological Sciences, Vol. 62, Number 2 (2012), 105-114


86.

A Comparative Study of Gastric Banding and Sleeve Gastrectomy in an Obese Diabetic Rat Model. T. Masuda., M. Ohta., T. Hirashita., Y. Kawano., H. Egucji., K. Yada., Y. Iwashita., S. Kitano. Obesity Surgery, Published online:27 August 2011


87.

Site dependency of fatty acid composition in adipose triacylglycerol in rats and its absence as a result of high-fat feeding. D. Sato., T. Nakamura., K. Tsutsumi., G. Shinzawa., T. Karimata., T. Okawa., Z. Fengc., and M. Kusunoki. Metabolism.Article in Press


88.

Dietary fructo-oligosaccharides improve insulin sensitivity along with the suppression of adipocytokine secretion from mesenteric fat cells in rats. A. Shinoki., and H. Hara. British Journal of Nutrition.Published online :02 June 2011.


89.

Food restriction improves glucose and lipid metabolism through Sirt1 expression: A study using a new rat model with obesity and severe hypertension. K. Takemori.,T. Kimura.,N. Shirasaka.,T. Inoue.,K. Masuno., and H. Ito. Life Sciences.Vol.88, Issues 25-26, 1088-1094. 2011


90.

Enhanced Urinary Bladder, Liver and Colon Carcinogenesis in Zucker Diabetic Fatty Rats in a Multiorgan Carcinogenesis Bioassay: Evidence for Mechanisms Involving Activation of PI3K Signaling and Impairment of P53 on Urinary Bladder Carcinogenesis. N. Ishii., M. Wei., A. Kakehashi., K. Doi., S. Yamano., M. Inaba., and H.Wanibuchi. Journal of Toxicologic Pathology .Vol. 24 (2011) , No. 1 pp.25


91.

Maternal low-protein diet suppresses vascular and renal endothelial nitric oxide synthase phosphorylation in rat offspring independent of a postnatal fructose diet. S. Sato.,Y. Mukai., and T. Norikura. Journal of Developmental Origins of Health and Disease (2011), 2: 168-175


92.

Combined Effects of Short-term Calorie Restriction and Exercise on Insulin Action in Normal Rats. H,Y,Jiang.,T,Koike.,P,Li.,Z,H,Wang.,Y,Kawata.,Y,Oshida. Horm Metab Res 2010; 42(13): 950-954


93.

Dietary Hesperidin Exerts Hypoglycemic and Hypolipidemic Effects in Streptozocin-Induce Marginal Type 1 Diabetic Rats. Akiyama,S., Katsumata,S., Suzuki,K., Ishimi,Y.,Wu,J., and Uehara,M.. J Clin Biochem Nutr.January;46(1):87-92.2010


94.

Hypoglycemic and Hypolipidemic Effects of Hesperidin and Cyclodextrin-Clathrated Hesperetin in Goto-Kakizaki Rats with Type 2 Diabetes. Akiyama,S., Katsumata,S., Suzuki,K., Nakayama,Y., Ishimi,Y. and Uehara,M. Bioscience,Biotechnology,and Biochemistry.Vol.73,No.12 pp.2779-2782(2009)

95.

Anti-Diabetic Effects of Pumpkin and Its Components,Trigonelline and Nicotinic Acid,on Goto-Kakizaki Rats. Yoshinari,O.,Sato,H.and Igarashi,K. Bioscience,Biotechnology,and Biochemistry.Vol.73,No5pp.1033-1041,2009


96.

Dietary Phosphatidylinositol Prevents the Development of Nonalcoholic Fatty Liver Disease in Zucker(fa/fa)Rats Shirouchi,B.,Nagao,K.,Inoue,N.,Furuya,K.,Koga,S.,Matsumoto,H. and Yanagita,T. J.Agric.Food Chem.56,2375-2379,2008


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Investigation of the anti-obesity action of licorice flavonoid oil in diet-induced obese rats. Kamisoyama,H.,Honda,K.,Tominaga,Y.,Yokota,S.,Hasegawa,S. Bioscience.Biotechnology and Biochemistry 72.(12)3225-3231,2008


98.

Erythrophagocytosis by Liver Macrophages(Kupffer Cells)Promotes Oxidative Stress, Inflammation,and Fibrosis in a Rabbit Model of Steatohepatitis. Otogawa,K.,Kinoshita,K.,Fujii,H.,Sakabe,M.,Shiga,R.,Nakatani,K.,Ikeda,K.,Nakajima,Y.,Ikura,Y.,Ueda,M., Arakawa,T.,Hato,F., and Kawada,N. American Journal of Pathology. 170:967-980, 2007


99.

Age- and sex-related diferences in spontaneous hemorrhage and fibrosis of the pancreatic islets in Sprague-Dawley rats. Imaoka, M., Satoh, H. and Furuhama, K. Toxicologic Pathology 35: 388-394, 2007

100.

Effect of 5-Campestenone (24-methylcholest-5-en-3-one) on Zucker Diabetic Fatty Rats as a Type 2 Diabetes Mellitus Model. R.Konno, Y.,Kaneko, K.,Suzuki, Y.,Matsui. Horm Metab Res ; 37(3): 79-83,2005

101.

Oxidized but not acetylated low-density lipoprotein reduces preproinsulin mRNA expression and secretion of insulin from HIT-T15 cells. Okajima,F.,Kurihara,M.,Ono,C.,Nakajima,Y.,Tanimura,K.,Sugihara,H.,Ttsuguchi,A.,Nakagawa,K., Miyazawa,T.,and Oikawa,S. Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids, Volume 1687, Issues 1-3, pp.173-180, 2005


102.

The role of calcium/calmodulin-dependent protein kinase cascade in glucose upregulation of insulin gene expression. Xiao Yu, Koji Murao, Yoshitaka Sayo, Hitomi Imachi, Wen M. Cao, Shouji Ohtsuka, Michio Niimi, Hiroshi Tokumitsu, Hiroyuki Inuzuka, Norman C.W. Wong, Ryoji Kobayashi, and Toshihiko Ishida. Diabetes, 53: 1475-1481, 2004


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PVA hydrogel sheet macroencapsulation for the bioartificial pancreas. Qi M, Gu Y, Sakata N, Kim D, Shirouzu Y, Yamamoto C, Hiura A, Sumi S, Inoue K. Biomaterials. 25: 5885-5892, 2004


104.

Alterations in vascular endothelial function in the aorta and mesenteric artery in type II diabetic rats. Takayuki Matsumoto, Kentaro Wakabayashi, Tsuneo Kobayashi, and Katsuo Kamata

Can. J. Physiol. Pharmacol. 82(3): 175-182, 2004


105.

Effect of eicosapentaenoic acid ethyl ester v. oleic acid-rich safflower oil on insulin resistance in type 2 diabetic model rats with hypertriacylglycerolaemia. Asako Minami, Noriko Ishimura, Sadaichi Sakamoto, Eiko Takishita, Kazuaki Mawatari, Kazuko Okada and Yutaka Nakaya. British J Nutrition 87, 157-162, 2002.


106.

Evaluation of Insulin Secretion of Isolated Rat Islets Cultured in Extracellular Matrix. Nagata N.; Gu Y.; Hori H.; Balamurugan A.N.; Touma M.; Kawakami Y.; Wang W.; Baba T.T.; Satake A.; Nozawa M.; Tabata Y.; Inoue K. Cell Transplantation, 10, 447-451, 2001


107.

Lactoferrin ameliorates corticosterone-related acute stress and hyperglycemia in rats. Maekawa Y, Sugiyama A, Takeuchi T. J Vet Med Sci. 2017 Feb 28;79(2):412-417.

产品编号 产品名称 产品规格 产品等级 产品价格
631-01479 (AKRIN-010T) Lbis® Rat Insulin ELISA Kit(T-type)
Lbis® 大鼠胰岛素 ELISA试剂盒(T型) 
96 tests