Shibayagi 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒

Shibayagi 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒
Lbis GLP-1(active) ELISA KIT

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Lbis GLP-1(active) ELISA KITShibayagi 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒

Shibayagi 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒

Shibayagi 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒

胰高血糖素样肽-1(Glucagon-like peptide-1,GLP-1)是胰高血糖素前驱体的一部分。胰高血糖素前驱体于胰脏、小肠下部以及下丘脑中表达。该前驱体的构造中含有与糖代谢有关的各种各样的生理活性物质(胰高血糖素,肠高血糖素,胃泌酸调节素,GLP-1,GLP-2)的氨基酸序列。根据表达部位加工酶的特异性,胰脏主要分泌胰高血糖素,而小肠下部主要分泌肠高血糖素和胃泌酸调节素。GLP-1和GLP-2则存在于胰高血糖素前驱体后半的结构中。GLP-1由37个氨基酸组成,有2种生物活性形式,分别为GLP-1(7-37)和GLP-1 (7-36)酰胺。两者都存在于小肠下部、胰脏和下丘脑中,GLP-1(7-36)酰胺在下丘脑中占免疫反应GLP-1(IR-GLP-1)总量的55-94%,在小肠中占27-73%。但在胰脏中只有极少量存在。大部分哺乳类(如人类、大鼠、小鼠、牛、猪、狗等)的GLP-1结构相似。

 

GLP-1: hdeferhaegtftsdvssylegqaakefiawlvkgrg  

GLP 1(7-37): haegtftsdvssylegqaakefiawlvkgrg

GLP 1(7-36) amide: haegtftsdvssylegqaakefiawlvkgr-NH2

 

GLP-1与小肠上部分泌的GIP统称为肠促胰素。该类激素是葡萄糖浓度依赖性方式促进胰岛素分泌。同时具有抑制胃肠道蠕动和胃液分泌、抑制胰高血糖素的释放、促进生长抑素的分泌、使食欲减退,促进肠上皮细胞生长、以及外周组织促进非胰岛素依赖性的葡萄糖的消耗,并促进细胞的生长的作用。有报告指出该类激素与垂体激素的分泌也有关系。

GLP-1(7-36)酰胺在生物体内代谢迅速,DPP-IV (dipeptidyl peptidase IV )会使其失去N-末端的两个氨基酸变为GLP-1(9-36)酰胺,GLP-1(7-37)变为GLP-1(9-37)后会失去活性。有报告指出,体外实验中,在犬的血浆中GLP-1(7-36)酰胺的半衰期是为61±9分,GLP-1(7-37)为132±16分。因此GLP-1的测定,取样的时候有必要使用DPP-IV抑制剂。

此外,肠促胰素中的GIP则是最有力促进GLP-1分泌的激素。回肠中GLP-1的分泌不是食物直接刺激肠道而产生的,而是由于胆碱能和肽类的刺激所产生的。

◆特点

● 短时间测定(完全反应时间:5小时)

● 微量样品(标准操作用量:10 μl)可测

● 使用对环境无害的防腐剂

● 全部试剂均为液体,可直接使用

● 精密的测定精度和高再现性

◆构成

 

组成

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96   wells(8×12)/1 块

(B) GLP-1标准溶液(500 pg/mL

稀释后使用

200 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D)生物素结合抗GLP-1抗体

稀释后使用

100 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

100 μL/1 瓶

(F) 显色液(TMB)

即用

12 mL/1 瓶

(H) 反应终止液(1M H2SO4)※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液(10×)

稀释后使用

100 mL/1 瓶

封板膜

4 张

使用说明书

1 份

◆交叉反应

※交差率是1,000 pg/mL浓度时的数据

动物类型

对象物质

反应性和反应率(%)

Mouse/Rat

GLP-1(7-36)amide

100

GLP-1(7-37)

<   0.1

GLP-1(1-37)

GLP-1(9-36)amide

GLP-2

Glucagon(1-29)

Insulin

Secretin

GIP

VIP

GRF

Bovine

Glucagon(1-29)

VIP

Porcine

Glucagon(1-29)

VIP

―:不存在交叉反应

◆样品信息

 

小鼠和大鼠的血清及血浆

10 μL/well(标准操作方法)

※测量中由于酶(DPP-IV等)的影响,采血时请注意防止GLP-1(7-36)酰胺的分解,再使用。

 

 

◆测定范围

 

1.56~50.0 pg/mL 【0.47~15.16 pmol/L(分子量3298)】(标准曲线范围)

7.8~250 pg/mL(样品量10 μ)

3.9~125 pg/mL(样品量20 μ)

◆Validation data

 

精度测试(检测内变异系数)

 

样品

A

B

1

23.7

6.44

2

23.2

5.97

3

23.4

6.39

4

24.0

5.87

5

24.1

6.44

mean

23.7

6.22

SD

0.35

0.28

CV(%)

1.5

4.5

单位:pg/mL

 

 

再现性测试(检测内变异系数)

 

测量日/样品

E

F

第0天

25.1

6.31

第1天

25.1

6.16

第2天

25.0

6.24

第3天

25.0

6.37

mean

25.0

6.27

SD

0.03

0.09

CV(%)

0.13

1.4

单位:pg/mL n=4

 

 

添加回收测试

 

样品C

添加量

实测值

回收量

回收率(%)

0.00

3.93

3.26

7.28

3.35

103

6.51

10.3

6.37

97.8

8.14

12.1

8.17

100

 

样品D

添加量

实测值

回收量

回收率(%)

0.00

11.8

7.16

19.1

7.30

102

14.3

25.5

13.7

95.8

21.5

32.4

20.6

95.8

 

 

稀释直线性测试

 

用稀释缓冲液分三次连续稀释2个血清样品的结果,直线回归方程的R2在0.997~0.9999之间。

相关资料


Shibayagi 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒 Shibayagi 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒

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产品编号 产品名称 产品规格 产品等级 产品价格
637-15129 (AKMGP-011) Lbis® GLP-1(Active) ELISA Kit
Lbis® 胰高血糖素样肽-1(GLP-1)(活性) ELISA试剂盒 
96 tests